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产品中心DNA Quantitation KitsBiotium/AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 7 DNA Standards/31028-T/trial-size 美国Biotium 公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的 BAY AREA------世界上首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其它特殊生化产品，如Fluo-3, Fura-2, BCECF，JC-1等。其产品被广泛应用于细胞生物学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模筛选等众多领域。 AccuClear™UltraHighSensitivitydsDNAQuantitationSolutionutilizesanovel,next-generationDNAbindingdyeforhighlysensitiveandaccuratequantitationofpurifieddsDNAsamplesacrossabroadrangeofDNAconcentrations.Theassayislinearbetween0.03ngand250ngofdsDNAperassayinmicroplateformatmakingitthemostversatileDNAquantitationassay.AccuClear™UltraHighSensitivitydsDNAQuantitationSolutionutilizesanovel,next-generationDNAbindingdyeforhighlysensitiveandaccuratequantitationofpurifieddsDNAsamplesacrossabroadrangeofDNAconcentrations.Theassayislinearbetween0.03ngand250ngofdsDNAperassayinmicroplateformatmakingitthemostversatileDNAquantitationassay.Unlikeabsorbance-basedmeasurements,AccuClear™dyeishighlyselectivefordouble-strandedDNAoversinglestrandedDNAorRNA.
TheAccuClear™dsDNAquantitationassayisdesignedforusewithfluorescence96-wellplatereadersequippedwithexcitationandemissionfiltersfordetectinggreenfluorescence.TheuniquespectralpropertiesofAccuClear™dyemakeitespeciallywell-suitedforusewithinstrumentswithblueLEDexcitationsources.AccuClear™alsoiscompatIBLewithhandheldfluorometerssuchasInvitrogen’squbit®andPromega’sQuantiFluor®-P,howeverthestandardcurvecalibrationprogramsfortheseinstrumentsmaynotcoverthefulldynamicrangeoftheAccuClear™kitstandardcurve. ForDNAquantitationusingtheQubit®werecommendourAccuGreen™HighSensitivitydsDNAQuantitationKit(catalogno.31066),whichwasformulatedspecificallyforthatinstrument.
TheAccuClear™UltraHighSensitivitydsDNAQuantiationKitwith7DNAStandardscontainsAccuClear™dye,1Xassaybuffer,andasetofsevencalfthymusdsDNAstandards.Note:thetrialsizekitincludesoneDNAstandardsolutionwithinstructionsforpreparingasetofstandardsbyserialdilution.Forlargernumbersofassays,BiotiumalsoofferstheAccuClear™UltraHighSensitivitydsDNAQuantiationKitwith1DNAStandard(2000assays,catalogno.31029),whichincludesAccuClear™dye,20Xassaybuffer,andacalfthymusdsDNAstandardsolutionforpreparingaseriesofstandardsbyserialdilution.AccuClear™UltraHighSensitivitydsDNAQuantitationSolution(1000assays,catalogno.31027),whichincludesdyeand1Xbufferwithoutstandards,isavailableforcustomerswhowishtopreparetheirownDNAstandards.
Components
AccuClear™Dye(100X)AccuClear™Buffer(1X)7CalfThymusdsDNAStandardsFeatures
Linearrange:0.03-250ngdsDNAGreenfluorescence(Ex/Em460/507nm)UnrivaledsensitivityanddynamicrangeNovelgreenfluorescentdyeisaperfectmatchforblueLEDexcitationsourcesGreenfluorescencedetectioncompatiblewithfluorescencemicroplatereader,handheldfluorometer,orNanodrop®fluorospectrophotometerOtherDNAQuantitationKits:
dsDNAQuantitationKitLineardetectionrange(dsDNAinassay)Ex/Em(nm)FeaturesAccuBlue NextGen1pg*-3ng468/507Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity.AccuClear UltraHighSensitivity30pg-250ng468/507Thiskitoffersgreatsensitivityandthewidestlinearrange.AccuBlue HighSensitivity0.2-100ng485/530QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye.AccuGreen HighSensitivity0.1-100ng502/523QuantitationofDNAusingtheQubit fluorometerfromThermoFisher.AccuBlue BroadRange2-2000ng350/460ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity.AccuClearkitsareintendedforquantitationofpurifieddsDNAsamples.Forcellquantitationassays,pleaseseeourcellviABIlityandcytotoxicityassays.
AccuClear™technologyiscoveredbygrantedU.S.andinternationalpatents.
Referencesn/a
InnovationAt Biotium, innovation is at the very heart of what we do every day. As a small technology company, we understand the importance of innovation to our competitiveness and growth. The company devotes a significant amount of resources to research and development and intellectual property protection. Our efforts have resulted in a growing number of granted and pending patents that cover a number of industry-leading technologies, including the following:
GelRed™ and GelGreen™ nucleic acid gel stains: highly sensitive DNA/RNA gel stains made safe by preventing dye penetration into cells
EvaGreen™ DNA-binding Dye: qPCR dye working on a novel \"release-on-demand” DNA-binding mechanism; the only dye that works perfectly for both qPCR and highly sensitive melt curve analysis
CF™ dyes: A series of 20 highly water soluble dyes with colors spanning the entire visible and near infrared spectra; designed for protein labeling using breakthrough chemistries to produce unrivaled performance in brightness and photostability
Mix-n-Stain™ Antibody Labeling Kits: A revolutionary protein labeling technology that allows anyone to optimally label an antibody , with or without the presence of BSA or gelatin, with one of our CF™ dyes or biotin in 30 minutes and ~100% yield.
NucView™ Caspase 3/7 Enzyme Substrate: A novel concept to detect caspase activity in live cells by releasing a cell nucleus-staining dye upon substrate cleavage
AccuClear™ and AccuBlue™ DNA Quantitation Kits: Kits with the lowest dsDNA detection limit and widest linear range in the market
PMA™ DNA Modification Agent: An essential reagent for selective detection of viable pathogens by qPCR
Product Quality and ServicesBiotium has state-of-the-art facilities and, more importantly, a team of highly experienced scientists and supporting staff to provide high quality products and superior service. Our chemistry and analytical labs employ advanced purification and analytical instruments to ensure product purity and consistency. Similarly, our bioscience lab is fully equipped for conducting cell imaging and bioanalytical experiments, such as confocal microscopy, gel electrophoresis, flow cytometry, real-time PCR, and fluorescent/bioluminescent plate-based assays. These capabilities enable us to produce user-friendly product protocols and provide superior technical support to our customers.
Business PartneringBiotium has licensed several of its patented technologies to a number of major international biotech companies including Sigma-Aldrich, Agilent, BioRad, Qiagen, BD Biosciences, EMD Millipore, Fluidigm, Essen BioScience, Caliper Life Sciences, Illumina, and others. Biotium’s products are sold world-wide through our international distributors, including VWR USA and VWR Europe. In addition, we frequently collaborate with academic scientists to develop new tools for the ever-changing needs in research. These partnerships extend Biotium’s reach to more customers and expand applications of Biotium’s cutting-edge technologies in life science research, medical diagnostics and pharmaceutical science.
