Anti-MED29抗体
| 产品编号 | YB-18768R |
| 英文名称 | MED29 |
| 中文名称 | MED29蛋白抗体 |
| 别名 | DKFZp434H247; Intersex like protein; IXL; Mediator complex subunit 29; Mediator of RNA polymerase II transcription subunit 29. |
| 规格价格 | 100ul/1380元购买200ul/2200元购买大包装/询价 |
| 说明书 | 100ul200ul |
| 研究领域 | 转录调节因子表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep, |
| 产品应用 | ELISA=1:500-1000IHC-P=1:400-800IHC-F=1:400-800ICC=1:100-500IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user. |
| 分子量 | 21kDa |
| 细胞定位 | 细胞核 |
| 性状 | Lyophilized or Liquid |
| 浓度 | 1mg/ml |
| 免疫原 | KLH conjugated synthetic peptide derived from human MED29: |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background:MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]Function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Subunit:Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.Subcellular Location:NucleusTissue Specificity:Widely expressed in embryo and adult.Similarity:Belongs to the Mediator complex subunit 29 family.SWISS:Q9NX70Gene ID:55588Database links: Entrez Gene: 614626Cow Entrez Gene: 55588Human Entrez Gene: 67224Mouse Entrez Gene: 292751Rat Omim: 612914Human SwissProt: A1A4Q8Cow SwissProt: Q9NX70Human SwissProt: Q9DB91Mouse Unigene: 611541Human Unigene: 41647Mouse Unigene: 23452Rat Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![](\"http://img.ebiomall.cn/images/sell/bs-18768R_13_small.jpg\") Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MED29) Polyclonal Antibody, Unconjugated (bs-18768R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining |
