您知悉并同意，丁香通平台展示产品并非为平台自身产品，您发起询价或试用申请后，平台将会把您已提供或按要求提供的相关信息同步提供至所咨询的具体产品商家，由商家为您提供具体产品的价格咨询或产品试用注意事项及相关试用产品或完成相应购买，因此您知悉并授权平台将您的信息进行同步共享。您有权拒绝，但您知悉将无法参加询价或试用活动。 RapiDxFire Thermostable Reverse Transcriptase 热稳定逆转录酶一种真正的热稳定逆转录酶，用于快速合成短cDNA（ 1 Kb） (30250-1  30250-2) 在高温下极其活跃（55-80°C）：通过各种RNA模板提高cDNA合成的特异性 敏感： 在两步RT-qPCR分析中检测≤100拷贝的RNA 反应时间短（5分钟或更短）： 简化的RT-qPCR工作流程和更快的出结果时间 在室温下稳定（ 3个月）：简化自动化卡座上的设置（无需冷藏），可在冷藏有限的环境中使用 Lyo兼容： 酶制剂不含甘油和其他成分，不会干扰下游冻干步骤 批次间重复性：在ISO 13485认证的设施中制造。产品包含RapiDxFireThermostable Reverse Transcriptase（3 Units /μL）和10XRapiDxFire Reaction Buffer。酶包装大小（50或250 Rxns）基于25μL反应体积。         RapiDxFire热稳定逆转录酶是一种显着优于流行的MMLV和AMV逆转录酶的方法。最初在温泉中发现，这种强大的噬菌体酶在升高的温度下表现出越来越高的活性（在90℃下10分钟后活性保持约60％）并且在20-25℃下储存 3个月后保持其核心活性。这种酶缺乏RNAse H和3\'→5\'核酸外切酶活性并有效合成短cDNA片段（≤1Kb）。与其他逆转录酶一样，RapiDxFire RT具有DNA聚合酶活性，但缺乏5\'→3\'核酸外切酶活性。酶的储存缓冲液不含甘油和Triton™X-100，可进一步优化用于下游冻干步骤。RapiDxFire耐高温RT在高温下表现最佳 Figure 1. Reaction temperature profile for RapiDxFireThermostable Reverse Transcriptase. Reverse transcriptionreactions were set up on ice using each manufacturer’s recommended buffersystem and Poly (rC) /p(dG)12-18 template/primer substrate. Reactions weretransferred from ice to the indicated temperatures (37, 50, 55, 60, 65, 70, 75,and 80°C) and incubated for 40 minutes. Following incubation, RNA/cDNAheteroduplex product is quantified as a measure of polymerization activity,utilizing PicoGreen® fluorescence on a Tecan Infinite® M1000 Pro. RapiDxFire ThermostableRT exhibits increasing activity as the reaction temperature is increased, up to80ºC (highest temperature tested); ~60% activity remains after 10 min at 90⁰C(data not shown). SuperiorDetection of Zika virus with RapiDxFire Thermostable RT(检测寨卡病毒的优异性) Figure 2. cDNA synthesistime course studies in a 2-step RT-qPCR reaction with different thermostableRTs. A) qPCR curve after a 1 minute reversetranscription reaction. Zika cDNA synthesis was conducted for each enzyme induplicate using target-specific primers and recommended reaction buffer andincubation temperatures per suppliers’ guidelines (RapiDxFire= 60⁰C, SupplierB= 50⁰C, and Supplier T= 55⁰C). After cDNA synthesis real time PCR wasperformed using one-tenth volume of each of the cDNA samples. PCR was performedusing EconoTaq and its supplied buffer (Lucigen Cat No. 30031-3). Detection ofPCR products were done real time using an intercalating dye (Dyomics Cat No. V13-01184)and BioRad CFX C1000 Touch™ with absorption/emission of 481nm/526nm. B) Reversetranscription time course study prior to performing second-step real-time PCR.Encircled data points are derived from data represented in the qPCR curve inA).3个月室温稳定性 图3. RapiDxFire热稳定性室温环境和-20°C下每个时间点单独存储活性对比。 在每个时间点RapiDxFire Thermostable RT通过测量10000拷贝MS2 RNA的第一链cDNA合成来评估。使用MS2的基因特异性引物在60℃的反应温度下进行cDNA合成5分钟。使用实时PCR和围绕520碱基对扩增子设计的MS2引物测量cDNA。产品名称： RapiDxFire Thermostable ReverseTranscriptase, 3Units/µL规格：50次， 250次 对应货号： 30250-1  30250-2欢迎来电咨询订购： 010-88891086       www.bjzblg.com     QQ:1443274331 温馨提示：不可用于临床治疗。 风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。