产品说明
PRODUCT SPECIFICATIONSITEM# 1081: Recombinant HIV-1 ADA Envelope Glycoprotein gp120 ( Baculovirus) Concentration: See vial Mass/vial See vial Diluent PBS Purity >98% Stabilizer None Preservative: None Storage: -75oC Physical State: Frozen Liquid Stability: At least 24 months at -75oC. DESCRIPTION: Gene cloned from M cell-tropic HIV-1 ADA strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System. PURIFICATION: This protein is purified by immuno-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA. BIOLOGICAL ACTIVITY: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti gp120 mAb-peroxidase capture ELISA. This protein activates human monocytic cells (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. APPLICATIONS CD4 Binding, Cell activation, In-Vitro Diagnostics.INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
ImmunoDX,LLC(IDX)-前身为ImmunoDiagnostics-为人类T细胞研究/诊断,艾滋病,HCV,HBV,TB,癌症和关节炎的诊断和治疗开发开发,制造和销售各种特种生物学。我们的独家产品系列-重组蛋白,单克隆/多克隆抗体和其他衍生试剂(FITC,过氧化物酶,生物素,顺磁性结合物)的生产符合纯度,特异性和生物活性的最高质量标准。
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