产品说明
Product Specifications:Item# 7001 Recombinant Human T-Cell Receptor sCD4 Concentration: See vial Mass/vial: 0.1mg Volume/vial: 1ml Diluent: PBS Purity: >98%Stabilizer: NonePreservative: NoneStorage: -75°CPhysical State: Frozen LiquidStability: At least 24 months at -75°CApplications: T-Cell Research,Human Diagnostics, HIV-1 Neutralization, Drug ScreeningDescription: Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4.Purification:This protein is purified by immunoaffinity and gel exclusion chromatography to >98% purity as determined by SDS PAGE, reduced.Molecular weight: Approximately 55kD.Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA.Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs.Application and Instructions for use:Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.1. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s).2. Recommended concentration for virus neutralization assays are in 1-10µg/ml of the HIV-1 infected culture.3. Drug screening assays are performed with 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate (catalog# 1121-P) or directly using HIV-1 rgp120-peroxidase conjugate (catalog# 1001-P).
ImmunoDX,LLC(IDX)-前身为ImmunoDiagnostics-为人类T细胞研究/诊断,艾滋病,HCV,HBV,TB,癌症和关节炎的诊断和治疗开发开发,制造和销售各种特种生物学。我们的独家产品系列-重组蛋白,单克隆/多克隆抗体和其他衍生试剂(FITC,过氧化物酶,生物素,顺磁性结合物)的生产符合纯度,特异性和生物活性的最高质量标准。
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