北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理，epicentre总代理，lucigen总代理...[查看全部]

ScriptCap™ Cap 1 Capping System C-SCCS1710

产品简介：北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理，epicentre总代理，lucigen总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。 /p

ScriptCap™ Cap 1 Capping System C-SCCS1710品牌介绍：

CELLSCRIPT专注于再生医学，细胞再生学以及细胞疗法。细胞、组织或器官的移植治疗及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。

ScriptCap™ Cap 1 Capping System C-SCCS1710规格详情名称：ScriptCap™ Cap 1 Capping System /p 分类：Post-Transcriptional Capping /p 货号：C-SCCS1710 /p 规格：10 reactions /p 产品详情：For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2\'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. /p 供应商：中北林格 /p 产地：us /p 发货地：北京 /p 运输方式：送货上门/顺丰 /p 是否现货：期货 /p

ScriptCap™ Cap 1 Capping System C-SCCS1710产品详情A. Synthesis of Cap 0 RNA from Uncapped RNA1. The protocol below was designed to build a cap 1 structure on 5\' end of 50-60 µg of uncapped RNA. If a cap 0 structure is desired, replace the ScriptCap 2\'-O-Methyltransferase in Step 3 with an equivalent volume of RNase-Free Water. Combine the following reagents:

Standard ScriptCap Cap 1 Capping Reaction (step 1)ComponentAmountRNase-Free Waterx µlIn vitro transcribed RNA, 50-60 µg≤67 µTotal Volume67 µl

2. Incubate at 65o C for 5-10 minutes, then transfer to ice.3. While the heat-denatured RNA is cooling on ice, prepare a \"Cocktail” of the following reaction components together in a separate tube.

Standard ScriptCap Cap 1 Capping Reaction (step 3)ComponentAmount10X ScriptCap Capping Buffer10 µl10 mM GTP10 µl20 mM SAM2.5 µlScriptGuard RNase Inhibitor2.5 µlScriptCap 2\'-O-Methyltransferase (100 U/µl)4 µlTotal Volume29 µl

4. Just prior to starting the reaction, add the ScriptCap Capping Enzyme to the Cocktail from Step 3 and then combine this with the uncapped RNA solution from Step 1.

Standard ScriptCap Cap 1 Capping Reaction (step 4)ComponentAmountCocktailed reaction components (from step 3)29 µlScriptCap Capping Enzyme (10 U/µl)4 µlHeat-denatured RNA (from step 1)67 µlTotal Reaction Volume100 µl

5. Incubate at 37o C for 30 minutes.6. The Capped RNA can now be purified, or it can be 3\' polyadenylated without purification by adding the reaction mixture directly to an A-Plus Poly(A) Polymerase Tailing Kit reaction (sold separately).

ScriptCap™ Cap 1 Capping System Contents (10 Reactions)Kit ComponentReagent VolumeScriptCap™ Capping Enzyme, 10 U/µl (red cap)40 µlScriptCap™ 2\'-O-Methyltransferase, 100 U/µl (blue cap)40 µl10X ScriptCap™ Capping Buffer. 0.5 M Tris-HCl, pH 8.0, 60 mM KCl and 12.5 mM MgCl2100 µl10 mM GTP Solution100 µl20 mM S-adenosyl-methionine (SAM)25 µlScriptGuard™ RNase Inhibitor, 40 U/µl25 µlRNase-Free Water1.4 ml

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ScriptCap™ Cap 1 Capping System C-SCCS1710 关键词：ScriptCap™ Cap 1 Capping System /p cellscript ScriptCap™ Cap 1 Capping System /p cellscript总代理 ScriptCap™ Cap 1 Capping System /p cellscript总代理中北林格 ScriptCap™ Cap 1 Capping System /p cellscript C-SCCS1710 10 reactions /p cellscript总代理 C-SCCS1710 10 reactions /p cellscript总代理中北林格 C-SCCS1710 10 reactions /p cellscript Post-Transcriptional Capping C-SCCS1710 /p cellscript总代理 Post-Transcriptional Capping C-SCCS1710 /p cellscript总代理中北林格 Post-Transcriptional Capping C-SCCS1710 /p

对此产品有兴趣，可联系中北林格获取更多详细信息。

主要用途 北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理，epicentre总代理，lucigen总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。