Description:
This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled). The molecular weightof ΦX174 RF I is 3.50 x 10
6 daltons and it is 5,386 base pairs in length. The vast majority of the molecules are RF I,and the remainder are RF II, as determined byagarose gel electrophoresis.DNASU and
Addgene are central repositories for plasmid clones and collections that may also be helpful.
Product Source:The ΦX174 RF I DNA is isolated from E. coli HF 4704infected with ΦX174 am3 cs70 by a modification of the method ofGodson and Vapnek (nucleotide sequenced by F. Sanger).
