Enzolifesciences/β-Catenin monoclonal antibody (dephospho, aa 35-50) (7A7)/ALX-804-259-C100/100
Product Specification:
| Alternative Name: | CTNNB |
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| Clone: | 7A7 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to aa 35-50 of human β-catenin conjugated to KLH. |
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| UniProt ID: | P35222 |
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| Species reactivity: | Human, MouseDog |
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| Crossreactivity: | Weakly cross-reacts with rat cytoplasmic dephospho β-catenin. |
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| Applications: | ELISA, ICC, IHC (PS), IP, WB |
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| Recommended Dilutions/Conditions: | ELISA (use at 0.05µg/ml)Immunocytochemistry (1-10µg/ml)Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. BSA may be used alternatively but milk is not recommended for blocking and dilution!)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
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| Positive Control: | Included. (Prod. No. ALX-840-022) |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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