Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BML-SA612-0200/200µl
Product Specification:
| Alternative Name: | Glutamate receptor 1, AMPA-selective glutamate receptor 1, Glutamate receptor ionontropic AMPA 1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 271-285 of rat GluR1 (GRIA1). |
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| UniProt ID: | P19490 |
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| Species reactivity: | Mouse, Rat |
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| Applications: | IHC, WB |
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| Recommended Dilutions/Conditions: | Immunohistochemistry (1:100)Western Blot (1:200)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
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| Blocking Peptide: | Included: GluR1 (extracellular) Control Peptide (100µg). Supplied lyophilized. |
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| Purity Detail: | Affinity purified |
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| Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide. |
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| Reconstitution: | To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. Further dilutions should be made using a carrier protein such as 1% BSA. To lyophilized control peptide, add 100µl of distilled water. |
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| Use/Stability: | Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | GluR receptors are permeable to cations Na and K and Ca2+. Gating of Glu receptors by glutamate is extremely fast and therefore the Glu receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. |
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| Technical Info/Product Notes: | PREADSORPTION CONTROL: 1µg control peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments. |
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