Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented. This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.查看细节    Western blot analysis of Synaptophysin was performed by loading 30 µg of Primary Rat Hippocampus neuron and Rat brain lysate (lane A, B) using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), Xcell SureLock™ Electrophoresis system (Product # EI0002), Novex sharp Pre-stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. Synaptophysin was detected at ~33 kDa using Synaptophysin Recombinant Rabbit Monoclonal Antibody (Product # 701503) at a 1:1000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).   Immunofluorescent analysis of Synaptophysin was done on Primary Rat Hippocampus neuronal cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Synaptophysin Recombinant Rabbit Monoclonal Antibody (Product # 701503) at a dilution of 1:400 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green).Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c is a merged image showing dendritic localization. Panel d is a no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.   Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-Synaptophysin Recombinant Rabbit Monoclonal Antibody (8H2L12) (Product # 701503), shows expression of Synaptophysin in SH-SY5Y cell line as compared to HeLa. {RE}   Fig.8 MO recovered memory-related proteins levels. A) Levels of PSD93, PSD95, Synapsin 1 and Synaptophysin were detected by western blotting in the hippocampus and beta-actin was used as loading control. B-E) Quantitative analysis of the blots showed that Hcy dramatically decreased the expression of these synaptic proteins in the hippocampus and either preventive or curative treatment with MO reversed these effects. The data were expressed as mean+-SD ( n = 6). # p 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96. Host server : magellan-srch-1-prod-green:8080/10.253.224.248:8080. git-commit: 2cd8645d2fc6bfe4ccb4abfa14772b0a94f68e98 git-url: http://victoria.invitrogen.com:8333/magellan/core.git git-branch: origin/release/1.27.0-2021.08.32-1.0