Capacity: for 100 preparations

Overview

The Neurosphere Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from cultured neurospheres. In less than an hour, the gentle enzymatic dissociation procedure efficiently yields high numbers of viable cells.Dissociated cells are immediately ready for further in vitro or in vivo applications such as cell culture and expansion, flow cytometry, fluorescence microscopy, and molecular applications, or for cell separations using MACS® Technology.

Details

Background information

Neurospheres are non-adherent spherical clusters of cells formed when neural stem cells are cultured under appropriate conditions. Effective dissociation is important for effective pasaging and subsequent downstream applicationsSome antigen epitopes are degraded by papain, others by trypsin. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, Anti-GLAST (ACSA-1) MicroBead Kit and Anti-PSA-NCAM MicroBeads require the use of the trypsin-based kit.Please see Figure 2 for further information with regard to epitope sensitivity and kit selection.