Capacity: for 2×10⁹ total cells

Overview

The Anti-Ly6B.2 (7/4) MicroBeads, mouse can be used for isolation of neutrophils, inflammatory monocytes or myeloid progenitors from bone marrow or spleen.

Details

Background information

• Positive selection or depletion of cells expressing mouse Ly-6B.2 antigen from bone marrow and spleen.
• Isolation of recruited inflammatory myeloid cells in experimental mouse models. The antigen is only expressed on mouse strains of the Ly6.2 haplotype.

Detailed separation procedure

First, the Ly-6B.2 (7/4)⁺ cells are magnetically labeled with Anti- Ly-6B.2 (7/4) MicroBeads. Then, the cell suspension is loaded onto a MACS^® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled Ly-6B.2 (7/4)⁺ cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of Ly-6B.2 (7/4)⁺ cells. After removing the column from the magnetic field, the retained Ly-6B.2 (7/4)⁺ cells can be eluted as the positively selected cell fraction. To increase the purity of spleen cells, the positively selected cell fraction containing the Ly-6B.2 (7/4)⁺ cells must be separated over a second column.

Columns

MS, LS, or autoMACS^® Columns.