Components:

• Anti-IL-4 pure – functional grade, mouse (130-095-709)
• Mouse IL-2, research grade (130-094-054)
• Mouse IL-12, research grade (130-096-707)

Overview

Starter kits for polarization of mouse T helper 1 cells, containing recombinant cytokines and blocking functional-grade antibodies.

Details

Background information

The different T helper (Tʜ) cell subsets have a central function in initiation, programming, and regulation of the various protective and pathological antigen-specific immune responses. T helper 1 (Tʜ1) cells are effective against intracellular pathogens and tumor cells and are also involved in immunological disorders, such as autoimmune reactions. In vitro, Tʜ1 cells can be generated from naive CD4⁺ T cells upon primary activation via T cell receptor in the presence of IL-12. Addition of IL-2 will enhance T cell proliferation. To inhibit polarization towards Tʜ2, the Anti-IL-4 pure – functional grade antibody is used.