24α-methyl Cholesterolagonist at liver X receptors (LXR) |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
- View current batch:
- Purity ≥ 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
24α-methyl Cholesterol Dilution Calculator
calculate
24α-methyl Cholesterol Molarity Calculator
calculate
Cas No. | 474-62-4 | SDF | Download SDF |
Synonyms | Campesterol,NSC 224330 | ||
Chemical Name | (3β,24R)-ergost-5-en-3-ol | ||
Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CC[C@@H](C)C(C)C | ||
Formula | C28H48O | M.Wt | 400.7 |
Solubility | ≤0.25mg/ml in ethanol;1mg/ml in dimethyl formamide | Storage | Store at -20°C |
Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
24α-methyl Cholesterol is an agonist of liver X receptors (LXR).
Liver X receptors (LXRs) are ligand-dependent transcription factors involved in the transcriptional control of lipid metabolism. LXRs function as nuclear cholesterol sensors that are activated by elevated intracellular cholesterol levels in multiple cell types. Activation of LXRs induces the expression of various genes involved in cholesterol absorption, transport, efflux, and excretion. In addition to their function in lipid metabolism, LXRs have also been found to modulate immune and inflammatory responses in macrophages. LXRα is highly expressed in the liver and at lower levels in the adrenal glands, adipose, intestine, lung, macrophages, and kidney, and LXRβ is ubiquitously expressed [2].
24α-methyl Cholesterol was a phytosterol found in vegetables, nuts, fruits, and seeds that competitively inhibited the absorption of intestinal cholesterol and decreased the transcription of genes important for cholesterol metabolism [3]. 24α-methyl Cholesterol acted as an agonist at liver X receptors (LXR) and suppressed the proliferation of prostate and breast cancer cells [1].
References:[1] Chuu C P, Kokontis J M, Hiipakka R A, et al. Modulation of liver X receptor signaling as novel therapy for prostate cancer[J]. Journal of biomedical science, 2007, 14(5): 543-553.[2] Zelcer N, Tontonoz P. Liver X receptors as integrators of metabolic and inflammatory signaling[J]. The Journal of clinical investigation, 2006, 116(3): 607-614.[3] Segura R, Javierre C, Lizarraga M A, et al. Other relevant components of nuts: phytosterols, folate and minerals[J]. British Journal of Nutrition, 2006, 96(S2): S36-S44.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
对于瞬时转染的检测:如果有报告基因,就通过报告基因的表达看转染成功与否;没有报告基因的话,就在24~96h内收获细胞通过RT-PCR和WB来鉴定。
由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
然后你得确定,这个细胞株对于你得研究来说不可或缺,具有明确的代表性。
上面两个都没有问题的话,祝你顺利。
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
推荐了解“主细胞库”“工作细胞库”这两个名词。
暂无品牌问答