Cat# | EG-1001(oldcatalog#:RP-7) |
Discount | $100DiscountCodeforYourFirstOrderof1mgCreRecombinase:CreDiscount Discountforswitchingfromcompetitors'product:$150.EmailtoTechSupportts@Excellgen.comfordiscountcode. |
Description | Crerecombinase,oftenabbreviatedtoCre,isaTypeItopoisomerasefromP1bacteriophagethatcatalyzessite-specificrecombinationofDNAbetweenloxPsites.Thisenzymedoesnotrequireanyenergycofactors,andCre-mediatedrecombinationquicklyreachesequilibriumbetweensubstrateandreactionproducts.TheloxPrecognitionelementisa34basepair(bp)sequencecomposedoftwo13bpinvertedrepeatsflankingan8bpspacerregionwhichconfersdirectionality.RecombinationproductsaredependentonthelocationandrelativeorientationoftheloxPsites.TwoDNAspeciescontainingsingleloxPsiteswillbefusedwhilstDNAbetweenloxPsitesinthesameorientationwillbeexcisedincircularformandDNAbetweenopposingloxPsiteswillbeinvertedwithrespecttotherestoftheDNA. Crerecombinaseisusedasatooltomodifygenesandchromosomes.InthisapproachtheCrerecombinaseisusedtodeleteasegmentofDNAflankedbyLoxPsites(aka'floxed')inanexperimentalanimal.Ithasbeenusedtogenerateanimalswithmutationslimitedtocertaincelltypes(tissue-specificknockout)oranimalswithmutationsthatcanbeactivatedbydrugadmiNISTration(inducIBLeknockout)inanumberoftransgenicspecies.TheavailABIlityoftransgeniclineswithtissuespecificorinducibleCreexpressionpermitsresearcherstoinactivateoractivateageneofinterestsimplybybreedingafloxedanimaltopre-existingCre-transgenics.OneexampleofaninducibleCrerecombinasesystemistheCre-ER(ER=EstrogenReceptor)systeminwhichintraperitonealinjectionoftamoxifenwillcausedose-dependentexcisionofthefloxedsite(i.e.willinactivatethegeneofchoice). RecombinantCrerecombinase(TAT-Cre)waspurifiedfromanE.colistraincarryinganengineeredplasmidencodingenhancedformofCreRecombinasefrombacteriophageP1.ThisCrerecombinasehasanN-terminal6XHistag,aTatpeptide(GRKKRRQRRRPPAGTSVSL)andanNLSsequence(PKKKRKV).HTNCisthemosteffectiveproteinintransduction(invivo)andsubsequentrecombinationcomparedtootherformsofCrerecombinases,e.g.,HNC,TCH6,HC,HNCM,CH.Incubationoffibroblastreportercellswith1μMHTNCfor1to2hourscanresultintranductionof60~90%ofthecells*.Additionof100μMchoroquinetoculturemediummay furtherenhancetransductionandrecombination. *Usethispagetocalculateconcentrationrequiredforcultured cells: http://www.excellgen.com/pub/mw_2_moles.html |
Applications |
|
Properties | Concentration:2,5,or10mg/ml. MolecularWeight:43kDa QC:Tat-Creinduced80~100%recombinationefficienciesinHEK293T-Crereportercells.HEK293T-cellsweretransfectedwithreporterplasmidDNA:LoxP-RFP-Stop-LoxP-GFP,thenadd1μMpurifiedTat-Creprotein. Fig1:0:beforeaddingTat-Cre;1to5:1to5daysaftertransductionwithTat-Cre. EndotoxinLevels:<0.1EU/μg Purity:>98%bySDS-PAGEandHPLCanalysis. |
UnitDefintion | Astandardof100UnitsisdefinedastheamountofTAT-CRE(μg) in1.0mLoftissueculturemediumthatisrequiredtoinduce50% GFPexpressioninaHEK293Treportercelllineassay. |
Stability | 2yearsat-80oC.6monthsat-20oC.Upto3weeksat4oC |
Citations |
|
Storage | -20ºC~-80ºC |
Shipping | dryice |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
grace粉末来配制,按说明书上的话加入碳酸氢钠外就不用加入
别的东西了,请问是否可以直接过滤用了:
在问,我们同一楼的另外一个实验室配制的时候加入了
水解乳蛋白和酵母粉,是否有这个必要,谢谢.在线,急等
不过因为食品添加剂种类很多,适应范畴和添加量都是规定的,如果你未按gb2760的规定使用,可能构成违法,具体是否是犯罪,要看具体的性质。
比如:二氧化钛(就是食品级的钛白粉)允许在允许在膨化食品、果冷、凉果等类限量使用,如果你在肉制品中使用,属于超范围墙使用,就是违法行为。
其次是超量使用,一般超量使用,又没有构成严重后果,属滥用食品添加剂,一般不按犯罪处理。
但超范围使用,加上媒体炒作的话,可能就麻烦了,因为公-安-执-法部门对此不太专业,往往会有意无意的扩大化,有可能会按刑法143条,生产销售伪劣食品罪起诉。
要注意:食品添加剂和非法添加物的区别,食品添加剂是国家允许使用的物质,而非法添加剂则是指食品及添加剂以外的物质,如三聚氰胺、吊白块、苏丹红等,不是食品添加剂,如果使用此类物质,则构成犯罪。
求助日本《医药品添加物事典》关于羟苯丙酯钠在口服溶液剂中最大用量
相容性药品包装材料与药物相容性试验指导原则2.pdf(43.35k)
暂无品牌分类
暂无品牌问答