Description
Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.
Cultrex®3DCultureMatrix®RGFBMEisaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.
Qualitycontrolspecifications:
- Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
- Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
- PathClear®–testednegativebyPCRfor17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- Proteinconcentrationof14–16mg/ml.
- NomycoplasmacontaminationdetectedbyPCR.
- ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
- GelstABIlitytestedforaperiodof14daysat37°C.
- BIOLOGicalactivitydeterminedforeachlot:
- Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
- 3DCulture:BasementMembraneExtractpromotesdifferentiationofahumanepithelialcelllinederivedfrommammarygland(MCF-10A)orhumanprostate(PC-3)intoacinarstructures.
Catalog#3445-010-01includes: | ||
CatalogNumber | Description | Qty |
3445-005-01 | 3-DCultureMatrix™ReducedGrowthFactorBasementMembraneExtract,PathClear® | 2 |
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grace粉末来配制,按说明书上的话加入碳酸氢钠外就不用加入
别的东西了,请问是否可以直接过滤用了:
在问,我们同一楼的另外一个实验室配制的时候加入了
水解乳蛋白和酵母粉,是否有这个必要,谢谢.在线,急等
不过因为食品添加剂种类很多,适应范畴和添加量都是规定的,如果你未按gb2760的规定使用,可能构成违法,具体是否是犯罪,要看具体的性质。
比如:二氧化钛(就是食品级的钛白粉)允许在允许在膨化食品、果冷、凉果等类限量使用,如果你在肉制品中使用,属于超范围墙使用,就是违法行为。
其次是超量使用,一般超量使用,又没有构成严重后果,属滥用食品添加剂,一般不按犯罪处理。
但超范围使用,加上媒体炒作的话,可能就麻烦了,因为公-安-执-法部门对此不太专业,往往会有意无意的扩大化,有可能会按刑法143条,生产销售伪劣食品罪起诉。
要注意:食品添加剂和非法添加物的区别,食品添加剂是国家允许使用的物质,而非法添加剂则是指食品及添加剂以外的物质,如三聚氰胺、吊白块、苏丹红等,不是食品添加剂,如果使用此类物质,则构成犯罪。
求助日本《医药品添加物事典》关于羟苯丙酯钠在口服溶液剂中最大用量
相容性药品包装材料与药物相容性试验指导原则2.pdf(43.35k)
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