Recombinant Human ACE2-His-Avi Protein (rhACE2-His)
Source: recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: Q9BYF1Predicted molecular mass: 86.5 kDa. Due to glycosylation, the recombinant human ACE2 protein migrates to 95-110 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 1 EU per ug by the LAL method.Formulation: The recombinant human ACE2-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
ACE2 (Angiotensin I Converting Enzyme 2) belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The SARS-CoV and SARS-CoV-2 (2019-nCoV) spike (S) proteins mediate viral entry into host cells by binding to a host receptor, ACE2, through the receptor-binding domain (RBD) of the S1 subunit, and then fusing the viral and host membranes through the S2 subunit.
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做ELISA实验，在处理样本前应该先了解收集样本的注意事项。
【标本收集注意事项】
1.收集血液的试管应为一次性的无热原，无内毒素试管。
2.血清和血浆避免使用溶血，高血脂标本。
3.标本应清澈透明，悬浮物应离心去除。
4.标本收集后若不及时检测，需按一次使用量分装，冻存于-20℃，-80℃电冰箱内，避免反复冻融。
5.可根据标本的实际情况，做适当倍数稀释(建议做预实验，以确定稀释倍数) 。
6.收集标本，尽量做到双份的用量，避免一次实验失败，重复实验时标本缺失，从而耽误实验进程。
7.收集标本时，应该做好防护措施（比如戴手套，口罩，护目镜等），认为所有标本都具有一定的潜在危险性。
8.样本处理应该在生物安全柜里面，并且正确使用生物安全柜。
样本正确的处理方法应该是这样的：
【标本处理办法】
1.血清：将采集的全血静置冰箱4℃过夜，然后1000-3000rpm离心10分钟，取上清立即测试，暂时不测可以放入-20℃（1-3个月）或-80℃（3-6个月）保存。
2.血浆：用EDTA，枸橼酸钠，肝素等作为抗凝剂，加入血液混匀后，1000-3000rpm离
心10分钟，取上清立即测试，暂时不测可以放入-20℃（1-3个月）或-80℃（3-6个月）
保存。
3.组织匀浆：切取组织块，0.01MPBS中过洗一次；按照1G组织加入5-10ml组织蛋白萃取试剂的比例，在冰水中匀浆。匀浆完成后，5000-10000rpm离心10分钟，取上清立即测试，暂时不测可以放入-20℃（1-3个月）或-80℃（3-6个月）保存。
4.细胞培养上清：1000-3000rpm离心10分钟，取上清立即测试，暂时不测可以放入-20℃（1-3个月）或-80℃（3-6个月）保存。
5.尿液，腹水，脑脊液等：1000-3000rpm离心10分钟，取上清立即测试，暂时不测可以放入-20℃（1-3个月）或-80℃3-6个月）保存。
注：样品稀释的一般原则
用户须查阅相关文献了解标本内待测因子的含量，决定适当的稀释倍数，以使稀释后样品中待测因子的浓度处于ELISA试剂盒的最佳检测范围。样品的稀释应有详细记录。
希望可以给您提供帮助，祝您科研顺利！
2×miRcute。
吹打最好还是用弯头的，用起来更为方便
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