VGlut2TransporterMonoclonalAntibody
CatalogNo.:56515(cloneS29-29)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.
PurifiedbyProteinGaffinitychromatography.
TheATP-dependent,chloride-sensitivevesicularglutamatetransportersincludeVGlut1,VGlut2,andVGlut3.VGlut1andVGlut2areexpressedinbraininacomplementaryfashion.VGlut1isexpressedinthetelencephalicregionswhereasVGlut2isexpressedinthelowerbrainstemanddiencephalicregions.
VGlut3isexpressedinasmallnumberofneuronsinthestriatum,hippocampus,cerebralcortex,andraphenuclei.
PancreaticαandβcellsexpressVGlut1andVGlut2inresponsetoglucoseconcentrations.
Antigen:Fusionproteincorrespondingtoaa501-582(cytoplasmicC-terminus)ofratVGlut2(accessionno.NP_445879).
HostSpecies:Mouse
AntibodyClass:IgG1
Thisantibodyrecognizeshuman,mouse,andratVGlut2.
APPLICATIONSImmunoblotting:useat1-2ug/ml.Abandof~60kDaisdetected.
Adultratbrainimmunoblot
Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:ratbrainlysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisproductisstableforatleast1yearat
-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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细胞质中有各种细胞器,细胞内的化学反应大多都是在细胞质中进行,因此是代谢中心.
细胞核中有DNA,是遗传信息库,是细胞代谢和遗传的控制中心.
解析:1.细胞质基质(也叫胞质溶胶)是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是:细胞核、线粒体基质、叶绿体基质等基质。
不破坏细胞结构,同步动态侦测细胞有氧呼吸,糖酵解OCR/ECA(总或乳酸ECA);
i.过氧敏感荧光素(或pH敏感荧光素),Ex340-380/535/Em630-680nm,实时测量线粒体/胞内/胞外重要代谢指标,荧光素为非结构性结合可逆转改变;
ii.可同时或单个测量OCR/ECA或其他参数,不会做成浪费,可采用时间分辨荧光技术可加强系统信噪比
iii.通过两个加药口,可对检测细胞加入适当抑制剂/刺激实时监测细胞对不同毒素作用
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