Highlights
- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description
Applicable For | Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications |
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Elution Volume | ≥ 10 µl |
Equipment | Microcentrifuge & Heat Block/Bath |
Processing Volume | ≤ 1.5 ml of Culture |
Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
Size Range | Up to 23 kb. |
Yield | Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures |
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Q2: What is the typical plasmid yield?
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.
Q3: Can this kit be used to isolate linear plasmid DNA?
Yes
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Q5: What yeast strains are these kits compatible with?
Any strains susceptible to Zymolyase, which includes:Susceptible fungal genera:AsbyaKloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis
Cat # | Name | Size | Price | |
---|---|---|---|---|
E1004 | Zymolyase | 1000 U | $75.00 | |
D2004-1-10 | Solution 1 Digestion Buffer | 10 ml | $18.00 | |
D2004-2-10 | Solution 2 Lysis Buffer | 10 ml | $18.00 | |
D2004-3-20 | Solution 3 Neutralizing Buffer | 20 ml | $18.00 | |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 |
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(1)移液杆可360旋转,不用工具可卸下121℃高温灭菌。
(2)符合人体工程学的外型设计。
(3)符合ISO9000和GLP规定,用户可独立校准。数字可调式移液器校准不需要工具。同时有较高的精度和误差系数。
(4)吸头接嘴有独特的V形圈,可以适合不同品牌的吸头。
移液器应用范围:
临床诊断实验室,生物技术实验室,药学和化学实验室,环境实验室,食品实验室
1.产品性能,即移液器的准确性和重复性对于绝大多数用户而言,购买之前检测产品性能既有难度又无必要.因此,主要还是依据制造厂商提供的技术数据.但在这里还是要说明两点:其一,不要轻易相信卖家的口头承诺,一定要查阅制造商提供的书面材料;其二,在全球移液器市场上影响较大的品牌,如SOCOREX,EPPENDORF和GILSON等,其提供的技术数据可信度更高.
2.产品的可靠耐用这一方面,主要取决于移液器所用的材料.对于外壳,应当有较高的耐冲击性、耐腐蚀性和较低的导热性(如PVDF材质);对于活塞,目前市场上主要有不锈钢、陶瓷和塑料三种材质.不锈钢机械性能好、寿命长,只是不太适合用于强酸强碱的移液;陶瓷则有很高的耐腐蚀性,但机械性能较差.当然,优质的材料往往意味着更高的价格,所以需要综合考虑购买价格和寿命的因素.
3.产品的人体工程学设计主要可以考虑以下几点:
第一,完成一个移液循环拇指的移动距离短,意味着舒适度更高;
第二,比较相同量程的移液器完成一次排液(一定要按到底)所需的拇指用力,这是影响舒适性的关键,用力越少意味着长期使用造成手指损伤的风险越小;
第三,装卸吸头,同样是越省力越好; 第四,移液器的重量适中,过重会增加手的负担,但过轻也往往意味着材质可能差强人意; 第五,其它的辅助设计,如壳体的磨砂设计以及指钩设计,有助于进一步提高舒适性.
移液器又称移液枪,是一种用于定量转移液体的器具,被广泛用于生物、化学等领域。
现在市场上经常把移液器分为三大类:
高端产品:非常精确耐用,Eppendorf、Gilson、RAININ和BIOHIT等品牌;
中端产品:一般精确耐用,BRAND、NICHIRYO(立洋)、SOCOREX、FinnPipette、CAPP和Hamilton等;
低端产品:大龙等等国产品牌,好在便宜,可以经常换。
各大移液器官方旗舰店
Eppendof旗舰店
RAININ旗舰店(METTLER旗下品牌)
BIOHIT旗舰店
大龙专卖店
Finnpipette旗舰店(ThermoFisher旗下品牌)
邀请广大战友来谈论一下使用移液器的心得和选购经验··········
版主鱼小留言:
支持活动。
密封良好的移液器,只要调节机构经过校准并且可以稳定保持精度,就不必担心有问题。
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