Product Name | NFkB-p65(Phospho-Ser536) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Applications | WB IHC IF |
Reactivity | Hu Ms Rt |
Specificity | he antibody detects endogenous level of NF-kB p65 only when phosphorylated at serine 536. |
Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65. |
Target Name | NFkB-p65 |
Alternative Name | NFKB3; RELA; TF65; Transcription factor p65; p65 |
Modification | Phospho-Ser536 |
SDS-PAGE MW | 65kd |
Accession No | Q04206; NP_001138610.1 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use. |
Application Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Background
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.
Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501. Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.
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（1）移液杆可360旋转,不用工具可卸下121℃高温灭菌。
（2）符合人体工程学的外型设计。
（3）符合ISO9000和GLP规定，用户可独立校准。数字可调式移液器校准不需要工具。同时有较高的精度和误差系数。
（4）吸头接嘴有独特的V形圈，可以适合不同品牌的吸头。
移液器应用范围：
临床诊断实验室，生物技术实验室，药学和化学实验室，环境实验室，食品实验室
1．产品性能,即移液器的准确性和重复性对于绝大多数用户而言,购买之前检测产品性能既有难度又无必要.因此,主要还是依据制造厂商提供的技术数据.但在这里还是要说明两点：其一,不要轻易相信卖家的口头承诺,一定要查阅制造商提供的书面材料；其二,在全球移液器市场上影响较大的品牌,如SOCOREX,EPPENDORF和GILSON等,其提供的技术数据可信度更高.
2．产品的可靠耐用这一方面,主要取决于移液器所用的材料.对于外壳,应当有较高的耐冲击性、耐腐蚀性和较低的导热性（如PVDF材质）；对于活塞,目前市场上主要有不锈钢、陶瓷和塑料三种材质.不锈钢机械性能好、寿命长,只是不太适合用于强酸强碱的移液；陶瓷则有很高的耐腐蚀性,但机械性能较差.当然,优质的材料往往意味着更高的价格,所以需要综合考虑购买价格和寿命的因素.
3．产品的人体工程学设计主要可以考虑以下几点：
第一,完成一个移液循环拇指的移动距离短,意味着舒适度更高；
第二,比较相同量程的移液器完成一次排液（一定要按到底）所需的拇指用力,这是影响舒适性的关键,用力越少意味着长期使用造成手指损伤的风险越小；
第三,装卸吸头,同样是越省力越好； 第四,移液器的重量适中,过重会增加手的负担,但过轻也往往意味着材质可能差强人意； 第五,其它的辅助设计,如壳体的磨砂设计以及指钩设计,有助于进一步提高舒适性.
移液器又称移液枪，是一种用于定量转移液体的器具，被广泛用于生物、化学等领域。
现在市场上经常把移液器分为三大类：
高端产品：非常精确耐用，Eppendorf、Gilson、RAININ和BIOHIT等品牌；
中端产品：一般精确耐用，BRAND、NICHIRYO(立洋)、SOCOREX、FinnPipette、CAPP和Hamilton等；
低端产品：大龙等等国产品牌，好在便宜，可以经常换。
各大移液器官方旗舰店
Eppendof旗舰店
RAININ旗舰店（METTLER旗下品牌）
BIOHIT旗舰店
大龙专卖店
Finnpipette旗舰店（ThermoFisher旗下品牌）
邀请广大战友来谈论一下使用移液器的心得和选购经验··········
版主鱼小留言:
支持活动。
密封良好的移液器，只要调节机构经过校准并且可以稳定保持精度，就不必担心有问题。
