ELISA Kit DIY Materials for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)
CCL24; SCYA24; Ckb-6; Eotaxin-2; Chemokine C-C-Motif Ligand 24; Eosinophil chemotactic protein 2; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 24
- Product No.KSA695Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.62-1000pg/mL
- Applicable Sensitivity6.3pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM96T*596T*1096T*2096T*5096T*100
- FOBUS$ 907 For more details, please contact local distributors!US$ 1512 For more details, please contact local distributors!US$ 2722 For more details, please contact local distributors!US$ 5292 For more details, please contact local distributors!US$ 9072 For more details, please contact local distributors!
SPECIFITY of the ELISA Kit DIY Materials for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)
The Abs in the kit have high sensitivity and excellent specificity for detection of Myeloid Progenitor Inhibitory Factor 2 (MPIF2). No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service
Related products
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA695Mu02 | Recombinant Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA695Mu01 | Recombinant Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA695Mu01 | Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | WB; IHC; ICC; IP. |
PAA695Mu02 | Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | WB; IHC; ICC; IP. |
LAA695Mu81 | FITC-Linked Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | WB; IHC; ICC; IF. |
LAA695Mu71 | Biotin-Linked Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | WB; IHC; ICC. |
SEA695Mu | ELISA Kit for Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA695Mu | Mini Samples ELISA Kit for Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA695Mu | CLIA Kit for Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | Chemiluminescent immunoassay for Antigen Detection. |
KSA695Mu01 | ELISA Kit DIY Materials for Myeloid Progenitor Inhibitory Factor 2 (MPIF2) | Main materials for "Do It (ELISA Kit) Yourself". |
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1)按到第一档,垂直进入液面几毫米。
2)缓慢松开控制按钮,否则液体进入吸头过速会导致液体倒吸入移液器内部吸入体积减少。
3)打出液体时贴壁并有一定角度,先按到第一档,稍微停顿1s后,待剩余液体聚集后,再按到第二档将剩余液体全部压出。
二、黏稠或易挥发液体的移取
在移取黏稠或易挥发的液体时,很容易导致体积误差较大。为了提高移液准确性,建议采取以下方法:
1)移液前先用液体预湿吸头内部,即反复吸打液体几次使吸头预湿,吸液或排出液体时最好多停留几秒。尤其对于移取体积大的液体(如1000~1),建议将吸头预湿后再移取。
2)采用反相移液法:吸液时按到第二档,慢慢松开控制按钮,打液时按到第一档即可,部分液体残留在吸头内。
三、常见的错误操作
1)吸液时,移液器本身倾斜,导致移液不准确(应该垂直吸液,慢吸慢放)。
2)装配吸头时,用力过猛,导致吸头难以脱卸(无需用力过猛,选择与移液器匹配的吸头)。
3)平放带有残余液体吸头的移液器(应将移液器挂在移液器架上)。
4)用大量程的移液器移取小体积样品(应该选择合适量程范围的移液器)。
5)直接按到第二档吸液(应该按照上述标准方法操作)。
6)使用丙酮或强腐蚀性的液体清洗移液器(应该参照正确清洗方法操作)。向左转|向右转
换算
1微升 = 0.000 000 001立方米
1微升 = 0.000 001 立方分米
1微升 = 0.000 001 升
1微升 = 0.001 毫升
1微升 = 0.001 立方厘米
1微升 = 1 立方毫米
1微升 = 1 000 纳升
第一步,拨掉ika移液器吸头弹射器放在盛放盘内;
第二步,将ika移液器容量值调至最大,拧开白套筒与手柄之间的连接螺帽;
第三步,白套筒端口向下,轻轻提起手柄放入盛放盘内,按住活塞尾部,将白套筒端口向上,置于盛放盘上部,小心取出活塞组件,以免弄掉零件;
第四步,从活塞杆上或白套筒内取出密封圈,放入盛放盘内。
最后,安装ika移液器,安装移液器的步骤与拆卸正好相反。需要注意的是,密封圈的黑色部份朝向白套筒,白色部份朝向手柄。
密封良好的移液器,只要调节机构经过校准并且可以稳定保持精度,就不必担心有问题。
1.使用合适的吸头:
为确保更好的准确性和精度,建议移液量在吸头的35%-100%量程范围内。
2.吸头的安装:
对于大多数品牌的移液器,特别是多道移液器,安装吸头并非易事:为追求良好的密封性,需要将移液套柄插入吸头后,左右转动或前后摇动用力上紧。也有人会用移液器反复撞击吸头来上紧,但这样操作会导致吸头变形而影响精度,严重的则会损坏移液器,所以应当避免出现这样的操作。RAININ(瑞宁)的多道移液器没有O型环,配合有前挡点的吸头,只需轻压一下即可达致理想密封,实在是多道移液器使用者的福音。
3.吸头浸入角度和深度:
吸头浸入角度控制在倾斜20度之内,保持竖直为佳;吸头浸入深度建议如下所示:
移液器规格
吸头浸入深度
2µL和10 µL
1 mm
20µL和100 µL
2-3 mm
200µL和1000 µL
3-6 mm
5000 µL和10 mL
6-10 mm
4.吸头润洗:
对常温样品,吸头润洗有助于提高准确性;但是对于高温或低温样品,吸头润洗反而降低操作准确性,请使用者特别注意。
5.吸液速度:
移液操作应保持平顺、合适的吸液速度;过快的吸液速度容易造成样品进入套柄,带来活塞和密封圈的损伤以及样品的交叉污染。
建议:
1、移液时保持正确的姿势;不要时刻紧握移液器,使用带指钩的移液器帮助缓解手部疲劳;有可能的话经常换手操作。
2、定期检查移液器的密封状况,一旦发现密封老化或出现漏液,须及时更换密封圈。
3、每年对移液器进行1-2次校正(视使用频率而定)。
4、绝大多数移液器,在使用前和使用一段时间后,要给活塞涂上一层润滑油以保持密封性;而对于RAININ常规量程的移液器,不涂润滑油也同样拥有理想的密封性。
如仍有疑问,欢迎向企业知道提问。
一、国产移液器我们以大龙移液器为例,它具有以下特点:
1. 重量轻,使用轻巧便捷,可减少手部疲劳。
2. 量程准确。
3. 量程具有锁定装置,符合人体手型和手感
4. 重量轻,使用轻巧便捷,可减少手部疲劳。
5. 具有可拆卸式高温高压消毒。
6. 产品规格齐全,共有16种。
二、进口移液器我们以德国艾本德移液器为例,它具有以下特点:
1. 符合ISO9000和GLP规定,用户可独立校准。
2. 十种规格,包括从0.1ul-5ml的体积变化,满足常规的需要,同时精度和误差较高。
3. 移液杆可360旋转,不用工具可卸下121℃高温灭菌。
4. 吸头接嘴有独特的V形圈,可以适合不同品牌的吸头。
5. 十种规格,包括从0.1ul-5ml的体积变化,满足常规的需要,同时精度和误差较高。
通过以上介绍,相信用户对这两种移液器的区别也有一定的了解了。
暂无品牌问答