Type
Recombinant
Description
TGF-βfamilymembersarekeymodulatorsofcellproliferation,differentiation,matrixsynthesis,andapoptosis.Asimpliedbytheirname,BMPsinitiate,promote,andregulatethedevelopment,growth,andremodelingofboneandcartilage.Inadditiontothisrole,BMPsarealsoinvolvedinprenataldevelopmentandpostnatalgrowth,remodelingandmaintenanceofavarietyofothertissuesandorgans.BMP-3 isabundantlyfoundinadultboneand,toalesserextent,fetalcartilage.BMP-3 inhibitsosteogenesisandboneformationbyactivatingasignalingcascadethatantagonizesthesignalingofpro-osteogenicBMPs.RecombinantHumanBMP-3 isadisulfide-linkedhomodimericproteinthatcorrespondstoresidues361 to472 ofthe472 aminoacidBMP-3 precursorprotein.ThecalculatedmolecularweightofRecombinantHumanBMP-3 is25.2 kDa.
AminoAcidSequence
MQWIEPRNCARRYLKVDFADIGWSEWIISPKSFDAYYCSGACQFPMPKSLKPSNHATIQSIVRAVGVVPGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVESCACR
Source
E.coli
Purity
95%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibitBMP-2-inducedalkalinephosphataseproductionbyATDC-5 cells.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C