Type
Recombinant
Description
BreastandKidney-expressedchemokine(BRAK)isaCXCchemokineexpressedinnormaltissueintheabsenceofinflammatorystimuli,andinfrequentlyexpressedincancercelllines.BRAKisknowntobeahighlyselectivemonocytechemoattractant.However,mainfunctionandreceptorselectivityisunknownatthistime.BRAKcontainsthefourhighlyconservedcysteineresiduespresentinCXCchemokines.Thesequenceofthematureproteinconsistsof87 aminoacidresidues,andisapproximately30%homologoustothesequencesofMIP-2 αandβ.RecombinantHumanBRAKisa9.4 kDaproteincontaining77 aminoacidresidues.
AminoAcidSequence
SKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRVYEE
Source
E.coli
Purity
98%
BIOLOGicalActivity
Determinedbyit"s ABIlitytochemoattractactivatedmonocytesusingaconcentrationrangeof1.0–10.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C