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Applied Biosystems™ POROS™ Prepacked Protein G, Affinity Column. POROS™ Protein G is based on an immobilized recombinant Protein G functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.
POROS™ High Performance Chromatography™ Media and Pre-Packed Columns
A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation
Reduce time-consuming sample prep
You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.
Create novel assays for more efficient analysis
High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.
High Capacity, High Resolution, High Speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.
POROS™ High Performance Chromatography™ Media and Pre-Packed Columns
A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation
Reduce time-consuming sample prep
You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.
Create novel assays for more efficient analysis
High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.
High Capacity, High Resolution, High Speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com

公司简介

蚂蚁淘(www.ebiomall.cn)是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台,
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息,找到合适的产品后在蚂蚁淘下单,然后蚂蚁淘的海外买手进行跨境采购、
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2021-09-21
虽然亲和层析法是进行特异性抗体纯化的首选,然而有时这种纯化方式却非必需,或者无法实施进行。在大多数锖况下,运铁蛋白可发生共沉淀或共纯化,能用凝胶滤过层析去除之。 查看更多
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2021-09-06
重组的果蝇 ACF 的表达和纯化实验的相关实验步骤、实验技巧、实验protocol、实验经验及常见问题。<link rel="stylesheet" type="text/css" href="/ueditor/them... 查看更多
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Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimensional (2D) g 查看更多
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2021-08-04
抗体作为生物学上的一个有用工具已经超过一个世纪。 1 9 世 纪 7 0 年 代 , Emil vonBehring 和 Shibasaburo Kitasato⑴最先阐述了免疫球蛋白,从那时起,抗体在研究和商业应用方面的使用取得了前所未有的进展。抗体最初是作为治疗病毒性疾病的天然抗血清来使用的。现今,高度工程化的治疗性抗体已被用于治疗多种危及生命的疾病。这一章将介绍纯化抗体的几种方法,尤其是单克隆抗体的纯化,纯化后的单克隆抗体具有多种用途。本章还将介绍抗体纯化前后的鉴定学方法。本章中,我们实验室产生的数 查看更多
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2018-12-26
蛋白质在组织或细胞中一般都是以复杂的混合物形式存在,每种类型的细胞都含有成千种不同的蛋白质。蛋白质的分离和提纯工作是一项艰巨而繁重的任务,到目前为止,还没有一个单独的或一套现成的方法能把任何一种蛋白质从复杂的混合物中提取出来,但对任何一种蛋白质都有可能选择一套适当的分 查看更多
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2018-12-26
Isolation of live bacteria from adult insectsHoracio M Frydman , hfrydman@princeton.edu, Associate Researcher Manuscript: 2005-11-13631BJournal: NatureArticle 查看更多
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2021-08-28
蛋白质组学和结构基因组学需要这种蛋白质提取方法, 它能够允许同时将几百个蛋白质在不同的载体和宿主的组合上进行筛选 (Stevensetal.,2001)。在这种多因素平行筛选 (multiparallelscreen) 中鉴定出来的能够正确折叠的表达水平高的最佳组合被放大到毫克级规模来生产,以便获得纯品进行功能和结构分析。这种高通量(high-throughout,HT) 的蛋白质表达和纯化策略加速了能够在微量和大量规模上高效裂解细胞和提取蛋白质的试剂及仪器的发展和优化。 查看更多
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Joseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwestern Medical Center, Dall 查看更多
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2018-12-26
1 分离方法 采取何种分离纯化方法要由所提取的组织材料、所要提取物质的性质决定。对蛋白质、多肽提取分离常用的方法包括:盐析法、超滤法、凝胶过滤法、等电点沉淀法、离子交换层析、亲和层析、吸附层析、逆流分溶、酶解法等。这些方法常常组合到一起对特定的物质进行分离纯化,同时上 查看更多
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2018-12-26
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 查看更多
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2018-12-25
For amplification of cognate sequences from different organisms, or for "evolutionary PCR", one may increase the chances of getting product by designing 查看更多
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2021-08-11
抗体作为生物学上的一个有用工具已经超过一个世纪。 1 9 世 纪 7 0 年 代 , Emil vonBehring 和 Shibasaburo Kitasato⑴最先阐述了免疫球蛋白,从那时起,抗体在研究和商业应用方面的使用取得了前所未有的进展。抗体最初是作为治疗病毒性疾病的天然抗血清来使用的。现今,高度工程化的治疗性抗体已被用于治疗多种危及生命的疾病。这一章将介绍纯化抗体的几种方法,尤其是单克隆抗体的纯化,纯化后的单克隆抗体具有多种用途。本章还将介绍抗体纯化前后的鉴定学方法。本章中,我们实验室产生的数 查看更多
>
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蚂蚁淘的创始人兼CEO是钟定松先生,具有十年的从业经验,在业界享有良好的口碑;
Ebiomall是跨境直采平台,我们直接从厂家采购,自己的团队负责国际物流和清关,中间没有第三方,蚂蚁淘承诺所售产品仅为正品,假一罚十。
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商品咨询
抗体纯化方法介绍 实验方法123
echoloving3182021-07-26
想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!
【求助】新手求教coIP抗体纯化的问题!谢谢! 蛋白质和糖学技术...123
clearor2021-07-31
请问如何选择幽门螺杆菌的血清抗体试剂盒_问答详情_蛋白纯化试剂盒...123
hys2021-07-20
如何选择二抗? 生物科学 抗体/Elisa 论坛学术科研互动平台123
Gloriachan2016-11-28
哪个公司企业的ELISA试剂盒好,性价比高,服务好?如何选择elisa试剂盒...123
2021-07-25
Agrisera 品牌介绍及代理商/经销商名单 品牌专区 123
2017-10-03
【求购】镍柱蛋白纯化试剂盒_问答详情_蛋白纯化试剂盒_蛋白纯化_...123
qinhuan04252021-08-15
【二抗】二抗是什么_孵育时间_稀释液_标记_稀..._英文,elisa二抗西域123
『帀』2017-10-03
顺手采纳答案
一抗的英语是 resistance No.1,二抗resistance No.2.
一抗的英语是 resistance No.1,二抗resistance No.2.
国内ELISA试剂盒哪个品牌好?123
★鲁鲁修★H9d2017-10-03
ELISA试剂盒很多牌子都不错,关键看灵敏度、实验效果、技术支持以及实验经费来决定
问个,一抗二抗结合的问题(elisa)123
星辰之守望者2017-10-03
质粒DNA提取和纯化试剂盒/质粒DNA提取和纯化试剂盒/质粒DNA...123
炎汐30082021-08-03
DNA纯化试剂盒与质粒DNA提取试剂盒这2者之间有区别吗?
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
求助:冰冻切片做免疫荧光的一些问题?非常感谢 免疫学讨论版...123
shamoyecao2021-08-08
请教各位大咖,冰冻切片做免疫荧光染色二抗如果放37度l小时,切片会不会干掉,细节应该怎么做。如果二抗室温(现在室内13度)1小时够不够,我是二抗室温lh染了2次,都没有满意的荧光。还有病理科的老师说冰冻切片之前不能固定,我是直接新鲜组织OCT包埋后冰冻切片的


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