Microarray Panel | High density thyroid cancer and thyroid tissue microarray, containg 24 each cases of papillary carcinoma and follicular carcinoma, 12 undifferentiated carcinoma, and 9 thyroid tissue, triplicated cores per case | |
Cores | 207 | |
Cases | 69 | |
Row number | 13 | |
Column number | 16 | |
Core Diameter (mm) | 1 | |
Thickness (µm) | 5 | |
Tissue Array Type | FFPE | |
Species | Human | |
Applications | Routine histology procedures including Immunohistochemistry (IHC) and In Situ Hybridization (ISH), protocols which can be found at our support page. | |
Notes | 1. TMA slides were sectioned and stored at 4°C and may not be fresh cut, but still suitable for IHC. Please request fresh cut if experiment involves phospho-specific antibodies, RNA studies, FISH or ISH, etc. A minimum of 3 slides per TMA must be purchased to cover the cost of trimming for fresh sectioning.2. Most TMA slides were not coated with an extra layer of paraffin (tissue cores can be easily seen on the glass). To prevent tissue detachment during antigen retrieval, unbaked slides must be baked for at least 30 to 120 minutes at 60°C. before putting into xylene for de-paraffinization. Baked slides were sent out baked for 2 hours.In the following specsheet,“*” means invalid core; “-” means no applicable or negative in IHC markers. |
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移液管移液管有两种类型.一种只有一个刻度,只能量取一定体积的液体,如25mL.另一种上的刻度和滴定管类似,每小格也表示0.1mL.移液管使用时通常用吸球吸取液体。移液管是吸入溶液的,不是固定的,流出溶液是靠壁流出的;
心肌肌钙蛋白I,英文为CardiactroponinI,该试剂盒在POCT领域内几乎是每个厂家都必做的项目,也是多数医院科室都开展的收费项目,市场容量很大,全国一年下来保守估计有3千万人份。但有些厂家销售的特别好,有些厂家销售的很一般。这一是由于销售渠道所致、二是因为产品质量差导致。试剂盒质量差一是因为厂家自身调试工艺不太好,二是由于所选择的抗体质量不好所致。现在市面上有心肌肌钙蛋白I抗体的厂家不多,大概也就不超过10家吧,主要为进口品牌,如:HyTest、BBI、Biospacific、Medix,国产品牌为深圳菲鹏,杭州启泰。其中着重要说的是杭州启泰,这是一家成立7年的抗原、抗体研发公司,公司不大,但立足于自主研发、创新,最近新推出的cTnI抗体可以说是目前国内外最好的抗体,这也算是填补了国内该领域的空白。从事了该行业将近10年,发此贴也是真切希望国内的生物试剂类公司,都专注于研发、创新,做一些好的试剂出来。因为很多事情国内做的并不比国外差的,国内的生物同行请加油吧!
四字原则:吸 擦 调 放
吸:用吸耳球吸取液体,四指拿移液管,食指扣于移液管上口。
擦:吸取后用滤纸擦干管壁。
调:放松食指使液体流出,调至刻度线,按紧。
放:沿器壁放入所需容器。
垂直是为了保证最后只剩下半滴时,移液管不计算在内的体积。如果移液管上没有写“吹”字,那么最后半滴可以不要。
移液管
用来准确移取一定体积的溶液的量器。移液管是一种量出式仪器,只用来测量它所放出溶液的体积。它是一根中间有一膨大部分的细长玻璃管。其下端为尖嘴状,上端管颈处刻有一条标线,是所移取的准确体积的标志。
大家做溶出曲线稀释溶液时有某有再用移液枪?移液枪量取1ml的体积准确不?
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