One anti-microRNAlentiviector(5.0ug)andits ready-to-useanti-miRNA lentivirus(0.5ml),and One negativecontrolanti-miRNAlenitvector(5.0ug)anditscontrol lentivirus(0.5ml).
Theanti-miRNAwasexpressedunderan optionalTetracyclineinducIBLe humanH1promoter.Itcanbeusedasconstitutiveanti-miRNAexpressionwithoutanyinduction, orusedastetracyclineinducibleexpressionwhenthe Tet-repressorprotein ispresentinadvance.Theanti-miRNAlentivectoraslocontainscontainsa GFP-Puromycidin fusiondualMarkerunderRsvpromoter,whichallowstoselectthepositivecellsbyPuromycinantibioticselectionorby cellsortingvia GFP signal. See Anti-miRNAlentivirus manual formoredetailsabouttheanti-miRNAlentivector.
Orderwillbedeliveredwithin 7-10 businessdays.
Cat#: A-miRNAH1-GP,
Amounts:(1) 0.5mlofpre-madespecificanti-miRNAlentiviruswithtiter at~1×107 IFU/ml;
(2)0.5mlofpre-madenegativecontrolanti-miRNAlentiviruswithtiter at~1×107 IFU/ml;
Note:pleaseprovidethemiRNA IDduringcheckoutinthe“Comments”field,or emailus themiRNAtargetIDoritsmaturemiRNAsequencethatyouwanttoinhibitorrepress.
Reference:
KluiverJ,GibcusJH,HettingaC,AdemaA,etal.(2012)RapidGenerationofMicroRNASpongesforMicroRNAInhibition.PLoSONE7(1):e29275.
