| productinformation
| | Background | | UDP-glucosepyrophosphorylase(UGPase,UDPGP)E.C=2.7.7.9. isakeyenzymeofsynthesisofsucrose,celluloseandothersaccharides.TherearetwocytoplasmicisoformsofUGPase-A(whichshare94%identityonaminoacidlevel)andonechloroplasticUGPase-BisoforminArABIdopsisthalianawhichshareca.10-11%ofidentity(Kleczkowskietal.2011). | | Immunogen | | RecombinantUGPaseQ43772overexpressedandpurifiedfromE.coli | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Serum | | Format | | Lyophilized | | Quantity | | 50µl | | Reconstitution | | Forreconstitutionadd50µlofsterilewater. | | Storage | | Storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes. | | Testedapplications | | Immunolocalization(IL),WesternBlot(WB) | | Relatedproducts | | AS142813 |UDP-glucosepyrophosphorylase(cytoplasmMarker)(Hordeumvulgare)
ForcytoplasmicmarkerforChlamydomonasreinhardtii-pleasecheckNAB1 Collectionofantibodiestocarbohydratemetabolism Recommendedsecondaryantibody Plantproteinextractionbuffer Secondaryantibodies | | Additionalinformation | | Cellular[compartmentmarker]ofcytoplasm,UGPseisacytoplasmicproteinMartzetal.(2002) |
applicationinformation
| | Recommendeddilution | | 1:1000-1:3000 withECL(WB),1:1500(IL) | | Expected|apparentMW | | 51.6kDa | | Confirmedreactivity | | Arabidopsisthaliana,Brassicarapa,Capsicumannuum,Cucumissativus,Festucaarundinacea,Hordeumvulgare,Lycopersicumesculentum,Lycopersicumchilense,MalusxdomesticaBorkh.c.v.Fuji,,Marchantiapolymorpha,M.vaginalis,Nicotianatabacum,Oryzasativa,Piceaglauca,Populussp.,Solanumlycopersicum,Solanumtuberosum,Solanumsogarandinu,Triticumaestivum | | Predictedreactivity | | Brassicapekinensis,Capsellarubella,Glycinemax,Glycinesoja, Gossipiumhirsutum,Pinustaeda,Populustremula,Ricinuscommunis,Saccharumofficinarum,Zeamays,Vitisvinifera,Citrussinensis,Jatrophacurcas,Cucumismelo,Bambusaoldhamii,Eucalyptusgrandis,Theobromacacao,Gossypiumhirsutum,Sorghumbicolor,Amorpha fruticosa,Brachypodiumdistachyon, formorespecies,pleaseinquire
| | Notreactivein | | C.merolae,diatoms | | Additionalinformation | | Thisantibodydetectes1ngofUGPaseinawesternblotandreactswithbothcytosolicisoformsonlywhichhavesimilarMWofca.52kDainArabidopsisthaliana | | Selectedreferences | | Nageletal.(2017).ArabidopsisSH3P2isanubiquitin-bindingproteinthatfunctionstogetherwithESCRT-IandthedeubiquitylatingenzymeAMSH3.ProcNatlAcadSciUSA.2017Aug7.pii:201710866.doi:10.1073/pnas.1710866114.
Schalketal.(2017).SmallRNA-mediatedrepairofUV-inducedDNAlesionsbytheDNADAMAGE-BINDINGPROTEIN2andARGONAUTE1.ProcNatlAcadSciUSA.2017Mar21.pii:201618834.doi:10.1073/pnas.1618834114.
Castellanoetal.(2016).ApathogeniclongnoncodingRNAredesignstheepigeneticlandscapeoftheinfectedcellsbysubvertinghostHistoneDeacetylase6activity.NewPhytol.2016Sep;211(4):1311-22.doi:10.1111/nph.14001.Epub2016May12.
Hsuetal.(2016).Super-resolutionribosomeprofilingrevealsunannotatedtranslationeventsinArabidopsis.ProcNatlAcadSciUSA.2016Oct21.pii:201614788.
Liuetal.(2016).iTRAQ-basedquantitativeproteomicanalysisrevealstheroleofthetonoplastinfruitsenescence.JProteomics.2016Sep2;146:80-9.doi:10.1016/j.jprot.2016.06.031.
Banceletal.(2015).ProteomicApproachtoIdentifyNuclearProteinsinWheatGrain.JProteomeRes.2015Sep8.
Kolbetal.(2015).FYVE1isessentialforvacuolebiogenesisandintracellulartraffickinginArabidopsisthaliana.PlantPhysiol.2015Feb19.pii:pp.114.253377.
Rounisetal.(2014).SeededandParthenocarpicCherryTomatoFruitsExhibitSimilarSucrose,Glucose,andFructoseLevels,DespiteDissimilaritiesinUGPaseandSPSGeneExpressionandEnzymeActivity.J.PlantGrowthRegul.,July2014.(immunolocalization)
Komatsuetal.(2014).PhototropinEncodedbyaSingle-CopyGeneMediatesChloroplastPhotorelocationMovementsintheLiverwortMarchantiapolymorphaL.1.PlantPhysiol.2014Sep;166(1):411-27.doi:10.1104/pp.114.245100.Epub2014Aug5.
Fukayamaetal.(2014).NocturnalphosphorylationofphosphoenolpyruvatecarboxylaseintheleavesofhygrophyticC3monocots.Bioscience,BiotechnologyandBiochemistry.
Szabalaetal.(2014).AccumulationofacidicSK3dehydrinsinphloemcellsofcold-anddrought-stressedplantsoftheSolanaceae.Planta,Jan7. |
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A1-year-oldgreehousegrownplantwasdissectedintodifferenttissues,whichwerethenusedforenzymeassaysandimmunoblotanalyses.Equalamountsoftotalprotein(7.5μg)wereloadedoneachlane.SDS-PAGEwasrunona7.5%gel.ImmunoblotwasdoneusingAmershamPVDFtransfermembrane.PrimaryantibodiesagainstbarleyUGPasewereusedin1:1000dilution.Secondaryantibodies(AmershamECLRabbitIgG,HRP-LinkedWholeAntibodyfromdonkey)wereusedat1:10000. ff-femaleflower,mf-maleflower,yl-youngleaf,ml-matureleaf,sbk-stembark,sph-stemphloemandcambium,sxy-stemxylem,rxy-rootxylem 
15µgof total solubleprotein extractfromleavesandstemsof Solanumtuberosum(1), Solanumsogarandinum(2), Lycopersicumesculentum(3), Lycopersicumchilense (4),Arabidopsisthaliana(5),Cucumissativus(6), Festucaarundinacea (7),Nicotianatabacum(8)andCapsicumannuum(9) wereseparated on10%SDS-PAGEandblottedontonitrocellulose. Afterblockingwith5%milkinTBST,blotswereincubatedwiththeprimaryantibodyatadilutionof1:1500inTBSTfor1h atroomtemperature.Followingincubationandwashsteps,blotswereincubatedwithSIGMAsecondaryAnti-RabbitIgG,AlkalinePhosphataseConjugatefor1houratadilutionof1:40000.BlotsweredevelopedwiththealkalinephosphatasedetectionsystemusingNBT/BCIP(SIGMA). CourtesyofBartoszSzabala,InstituteofPlantGenetics,PolishAcademyofScience.
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