pCold expression vectors offer cold-shock expression technology for high-purity, high-yield protein production.
The pCold series includes several different vectors. Each includes the cold-shock Protein A (cspA) promoter for expression of high-purity, high-yield recombinant protein in E. coli. These vectors selectively induce target protein synthesis at a low temperature (15°C), a condition which suppresses the expression of host proteins and decreases protease activity. This results in high yields of target proteins (60% of intracellular protein). In addition to the cspA promoter, all vectors contain a lac operator, ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and a multiple cloning site (MCS). Four of the vectors contain either a translation enhancing element (TEE), his-tag sequence, and/or Factor Xa cleavage site. The pCold GST vector contains a glutathione S-transferase (GST) sequence and can be used for the expression of proteins with an N-terminal GST tag.These vectors work equally well for synthesis of non-labeled and radiolabeled proteins, and can be used in conjunction with our Chaperone Plasmid Set.
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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