Format : | Purified |
Amount : | 100 µg |
Isotype : | Rabbit IgG, kappa |
Purification : | Purified Ab with BSA and Azide at 200ug/ml |
Content : | 200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Gene : | CHGA |
Gene ID : | 1113 |
Uniprot ID : | P10645 |
Alternative Name : | Beta-Granin,CGA,CHGA,Chromogranin A Parathyroid Secretory Protein 1,ER-37,Pancreastatin,Parastatin,Parathyroid Secretory Protein 1,Pituitary Secretory Protein I,SP-I,Vasostatin I or II |
Immunogen Information : | Recombinant human full-length Chromogranin A protein |
Does not react with rat.
MW : 68-75kDa; Positive Control : PC12 cells. Adrenal gland, bowel, parathyroid, pancreas, or pheochromocytoma.;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Secreted, Cytoplasmic vesicle |
Post transnational modification: | Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725). |
Tissue Specificity: | GE-25 is found in the brain. |
BioGrid: | 107538.12 interactions. |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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