Product Name | Neurogranin 28 - 43 [AAKIQASFRGHMARKK], Biotinylated - LCBiotin - LC - AAKIQASFRGHMARKK |
Size | 5 mg |
Catalog # | AS-27176 |
US$ | $611 |
Purity | % Peak Area By HPLC ≥ 95% |
The native peptide AAKIQASFRGHMARKK (27173) is a synthetic PKC substrate derived from the sequence of neurogranin, a naturally occurring PKC substrate. The peptide is proven to be a PKC substrate with quite good specificity. It is phosphorylated by purified PKC with a Km of 150 nM. No significant phosphorylation of the peptide by either PKA or by CaMK 2 is reported. Substituting Arg36 with Ile causes a significant reduction in the affinity for PKC. Replacing Lys30 with Arg enhances the catalytic efficiency (Vmax/Km) for PKC but diminishes the selectivity of the substrate for PKC. It is generally considered that basic amino acids on both sides of the phosphorylated Ser are important structural determinants in PKC substrates. In addition, the presence of particular basic amino acids (Arg vs Lys) may also contribute to the degree of selectivity of a substrate for PKC. | |
Detailed Information | Material Safety Data Sheets (MSDS) |
Storage | -20°C |
References | Ref: Chang, DK. et al. Biophys. J. 72, 554 (1997); Chen, SJ. et al. Biochem. 32, 1032 (1993). |
Molecular Weight | 2139.6 |
Biotin-LC-AAKIQASFRGHMARKK | |
Sequence(Three-Letter Code) | Biotin - LC - Ala - Ala - Lys - Ile - Gln - Ala - Ser - Phe - Arg - Gly - His - Met - Ala - Arg - Lys - Lys - OH |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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