ProductHighlights:
TheNexteraDNAFlexLibraryPrepKitoffersflexibilityformanywhole-genomesequencingapplications.
- FastestIlluminalibraryprepworkflow,with~3.5hourstotaltime
- Flexibilitytoaccommodatevariationsinsampletype,DNAinputamount,andapplication
- Optimizedlibraryprepperformance,generatingreliableresults
SaveTimeandResources
TheNexteraDNAFlexLibraryPrepKitusesafast,user-friendlyworkflow.On-beadtagmentationchemistryreducestotallibrarypreptimeto~3.5hours,fromDNAextractiontolibrarynormalization.
SimplifyLabOperations
TheNexteraDNAFlexworkflowsupportsabroadDNAinputrange(1–500ng),multiplesampletypes,andbothsmallandlargegenomes.TheworkflowincludesDNAextractionfromblood,saliva,ordriedbloodspots*orbacterialcolonies*.Gaintheflexibilitytosequencehumanorotherlarge,complexgenomesaswellasampliconsormicrobialspecies,allwithasinglekit.
ObtainReliableResults
Whileaccommodatingvariousstudyrequirements,theNexteraDNAFlexworkflowdeliversconsistentinsertsizes,uniformcoverage,andoptimizedperformance,regardlessofDNAinputamountorgenomesize.Thebead-basedtechnologyminimizesbiasandopportunitiesforerror,resultinginhighlyreproducIBLesequencingdata.
*Demonstratedprotocolsavailable.
Specifications:
AssayTime | ~3.5hourstotalassaytime |
Hands-OnTime | 1-1.5hours |
InputQuantity | SmallGenomes:1-500ngDNA;LargeGenomes:100-500ng |
ContentSpecifications | HumanWholeGenome,SmallWholeGenome,LargeWholeGenome |
MechanismofAction | Bead-linkedtransposome |
Multiplexing | Upto96availableindexes |
SpeciesCategory | AnySpecies |
SystemCompatibility | MiSeq,HiSeqXTen,NextSeq550,HiSeq3000,HiSeqXFive,MiSeqDxinResearchMode,MiniSeq,HiSeq2500,NovaSeq6000,HiSeq4000 |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels),CopyNumberVariants(CNVs) |
Method | Whole-GenomeSequencing |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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