ProductHighlights:
FastLibraryPrep,OptimizedforSmallGenomes,PCRAmplicons,andPlasmids
- Rapidlibrarypreparation–completelibraryprepinaslittleas90minuteswithonly15minutesofhands-ontime
- Fasttimetoresults–gofromDNAtodatain8hourswithourbenchtopsequencers.
- Optimizedforsmallgenomes,PCRamplicons,andplasmids–onelibraryprepkitformanyapplications
- Innovativesamplenormalization–eliminatestheneedforlibraryquantificationbeforesamplepoolingandsequencing
WithNexteratechnology,DNAissimultaneouslyfragmentedandtaggedwithsequencingadaptersinasingletubeenzymaticreaction.NexteraXTsupportsultra-lowDNAinputofonly1ng.Itenablesawiderangeofinputsamples,including smallgenomes,PCRampliconsgreaterthan300bp,plasmids,microbialgenomes,concatenatedamplicons,anddouble-strandedCDNA.
Multiplexingofupto384samplesperNexteraXTlibraryisalsoavailableforthoseprojectsrequiringgreaterthroughput.Plus,thekitincludesaninnovativebead-basedsamplenormalizationthateliminatestheneedforlibraryquantificationpriortopoolingandsequencing.LibrariespreparedwithNexteraXTkitsarecompatIBLewithallIlluminasequencers.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
AssayTime | 90minutes |
Hands-OnTime | 15minutes |
MechanismofAction | EnzymaticFragmentation |
Multiplexing | Upto384uniquelyindexedsamplesmaybepooledandsequencedtogether. |
InputQuantity | 1ngDNA |
SpeciesCategory | Drosophila,AnySpecies,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria |
SystemCompatibility | MiSeq,NextSeq550,NextSeq500,MiSeqFGxinResearchMode |
Method | ShotgunSequencing,Whole-GenomeSequencing,DeNovoSequencing,16SrRNASequencing,AmpliconSequencing,TargetedDNASequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants |
SpecializedSampleTypes | LowInput,SingleCells |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
NexteraXTLibraryPreparationWorkflowimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:
ThecombinationofNexteraXTandrapidsequencingwiththeMiSeqSystemprovidesacompleteDNAtodataworkflowinonly8hours.InnovativeSampleNormalizationimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-2.pngebiomall.com
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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