ProductHighlights:
FastLibraryPrep,OptimizedforSmallGenomes,PCRAmplicons,andPlasmids
- Rapidlibrarypreparation–completelibraryprepinaslittleas90minuteswithonly15minutesofhands-ontime
- Fasttimetoresults–gofromDNAtodatain8hourswithourbenchtopsequencers.
- Optimizedforsmallgenomes,PCRamplicons,andplasmids–onelibraryprepkitformanyapplications
- Innovativesamplenormalization–eliminatestheneedforlibraryquantificationbeforesamplepoolingandsequencing
WithNexteratechnology,DNAissimultaneouslyfragmentedandtaggedwithsequencingadaptersinasingletubeenzymaticreaction.NexteraXTsupportsultra-lowDNAinputofonly1ng.Itenablesawiderangeofinputsamples,including smallgenomes,PCRampliconsgreaterthan300bp,plasmids,microbialgenomes,concatenatedamplicons,anddouble-strandedCDNA.
Multiplexingofupto384samplesperNexteraXTlibraryisalsoavailableforthoseprojectsrequiringgreaterthroughput.Plus,thekitincludesaninnovativebead-basedsamplenormalizationthateliminatestheneedforlibraryquantificationpriortopoolingandsequencing.LibrariespreparedwithNexteraXTkitsarecompatIBLewithallIlluminasequencers.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
AssayTime | 90minutes |
Hands-OnTime | 15minutes |
MechanismofAction | EnzymaticFragmentation |
Multiplexing | Upto384uniquelyindexedsamplesmaybepooledandsequencedtogether. |
InputQuantity | 1ngDNA |
SpeciesCategory | Drosophila,AnySpecies,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria |
SystemCompatibility | MiSeq,NextSeq550,NextSeq500,MiSeqFGxinResearchMode |
Method | ShotgunSequencing,Whole-GenomeSequencing,DeNovoSequencing,16SrRNASequencing,AmpliconSequencing,TargetedDNASequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants |
SpecializedSampleTypes | LowInput,SingleCells |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
NexteraXTLibraryPreparationWorkflowimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:
ThecombinationofNexteraXTandrapidsequencingwiththeMiSeqSystemprovidesacompleteDNAtodataworkflowinonly8hours.InnovativeSampleNormalizationimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-2.pngebiomall.com
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败,此外,由于采用了剧烈的玻璃珠击打来破裂菌体,常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA,通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱,可以最大程度的去除这些杂质,同时加上多次柱漂洗,确保得到的DNA具有极高纯度,此外独特的抽提和裂解体系可以迅速裂解细胞(壁)和灭活细胞内核酸酶,不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性。
产品特点:
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。
3.兼容性强,适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物,保证了极高纯度,OD260/OD280典型的比值达1.7~1.9。
5.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
6.快速,简捷,单个样品操作一般可在60分钟内完成。
你找下,杭州昊鑫生物
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
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