ProductHighlights:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitisascalableampliconsequencingsolutionthatdeliverssensitiveandspecificresultsfrombothlow-inputandformalin-fixed,paraffin-embedded(FFPE)DNAsamples.Itoffers:
- Accuratevariantdetectionfromaslowas10ngofinputgenomicDNA(gDNA)andchallengingFFPEsamples
- Fullysupported,optimizedworkflowsolutionincludingsimpledataanalysis
- Automation-friendlyworkflowthatcanbecompletedin6hours,withonly3hoursofhands-ontime
LowDNAInputandFFPECompatibility
TheTruSeqCustomAmpliconLowInputLibraryPrepKitisafullycustomizable,amplicon-basedassayfortargetedresequencingstartingfromaslowas10ngofgenomicDNA(gDNA).Thisscalableassayallowsresearcherstocapturemultipletargetsofinterestsimultaneouslyandsequenceupto1536ampliconsinasinglepool,usingasinglereaction.ThislibraryprepkitofferstheflexibilitytoaccommodateFFPEsamples,suchaspreservedtumortissue.
ConfidentAssayDesign
DesigntargetedTruSeqCustomAmpliconoligonucleotideprobeswithDesignStudio,afree,easy-to-useonlinetoolthatprovidesoptimizedcoverage.
LearnmoreaboutDesignStudio
StartaprojectinDesignStudionow(loginrequired)
AssessFFPESampleQualitybeforeSequencing
TheTruSeqFFPEDNALibraryPrepQCKitusesasimpleqPCRreactiontodetermineDNAqualityandprovideguidanceonsequencingparameters.Thisstepensuresthatonlysamplesthatwillachievethenecessarysequencingmetricsareprepared,conservingresourcesthatmightbeusedonpotentiallylow-quality,unrecoverablesamples.TheresultsoftheQCstepdeterminetherecommendedamountofinputDNA.TheTruSeqFFPEDNALibraryPrepQCKitcanbebundledwiththeTruSeqCustomAmpliconLowInputLibraryPrepKittomaximizelabbudgets.
ForFFPEapplications,wesuggestdesigningyourprojectwith150bpor175bpampliconssinceFFPEDNAishighlydegraded.TruSeqCustomAmpliconLowInputcannotbeusedwithapplicationsrequiring425bpamplicons.
SpeciesCompatibility
TheTruSeqCustomAmpliconLowInputLibraryPrepKitsupportshuman,mouse,rat,andbovinesamples.DesignStudiodesignalgorithmsarealsoavailableformaize,rice,pig,dog,soybean,chicken,andsheep;however,assayperformancecannotbeguaranteedastheresultingassayshavenotbeentested.Inaddition,IlluminaConciergeservicescandesigncustomassaysand/orprovideassayoptimizationsupportforanyspeciesofinterest.
InquireaboutIlluminaConciergeservices
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 4hours |
InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
Multiplexing | Upto384samplespersequencingrun |
MechanismofAction | Amplification |
TruSeqCustomAmpliconLibraryPrepProtocol:
TheTruSeqCustomAmpliconassayisasimpleandstreamlinedmethodforcapturingandamplifyingtargetedregionsofinterest.SeamlessAmpliconSequencingWorkflow:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitispartofanintegrated,fullysupportedworkflowforampliconsequencingthatguidesresearchersfromdesignthroughdataanalysis.Illuminatechnicalandfieldspecialistshelpensurerapidresolutionandminimizepotentiallaboratorydowntime.RobustVariantDetectionatLowDNAInput:
TheTruSeqCustomAmpliconLowInputLibraryPrepKitdemonstrateshighconcordancebetweenexpectedandobservedvariantfrequenciesfor10nghigh-qualityFFPEDNA.ReferenceDNAsampleswerepreparedfollowingtheTruSeqCustomAmpliconLowInputworkflowwiththeTruSeqAmplicon-CancerPanelprimerpool,andsequencedontheMiSeqSysteminreplicatesof4.VariantswerecalledusingMiSeqReporterSoftware.R2valuesareshown.ebiomall.com
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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