ProductHighlights:
- Unlocksmallsamples(50–100ngDNAinput)
- CpG,CHH,&CHGregionsincludedforcomprehensive,whole-genomeresults
- Fastprotocol–five-hourmethod
- Capturefullsamplediversity
Sequencetheentiresample–nolossofinformation
TheprocessofbisulfitetreatmentdenaturesgenomicDNAintosinglestrandedDNA.TruSeqDNAMethylationconvertssinglestrandedDNAintoanIlluminasequencinglibrary.AllssDNAfragmentsarecapturedintoanIlluminasequencinglibrary,thereforeeliminatingthelossofdiversityassociatedwithothermethods.
Supportedanalysisinthecloud
TruSeqDNAMethylationlibrariescanbealignedtothehumangenomeandcomparedfordifferentialmethylationwithIlluminaBaseSpaceAppsMethylSeqandMethylKit.TheseapplicationsarefullysupportedandweredevelopedspecificallyforTruSeqDNAMethylationlibrarypreparation.
ExampledatasetsforTruSeqDNAMethylationlibraries,alsoavailableinBaseSpaceSequenceHubDataCentral(usethe“MethylSeq”categoryfilter),demonstrateunparalleledqualityandseamlessanalysis.
Deepcoverageofcriticalgenomicregions
DepthofcoverageisenhancedingenomicareaswithBIOLOGicalutility.TruSeqDNAMethylationcapturesfullsamplediversityofcriticalareas,including:
- Codingregionstartandendforexonsfromthecanonicaltranscriptofproteincodinggenesforgenesknowntobeinvolvedincancer,takenfromSOMAandCRUKpanels(aswellasliterature-derivedcancergenes)
- GenesdefinedbytheAmericanCollegeofMedicalGeneticsasbeingmedicallyrelevant(ACMGgenes)
- ExoniccodingregionsfromEnsemble70
- Listof100promotersdefinedbytheBroadInstituteasbeingofhighinterestanddifficulttosequence
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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