ProductHighlights:
TheTruSeqNanoDNALibraryPreparationKitenablesefficientinterrogationofsampleswithlimitedavailableDNA.Basedontheindustry’smostwidelyadoptedlibrarypreparationworkflow,thislow-inputmethoddelivershighcoveragequalityandreducedbiasforvirtuallyanysequencingapplication.
- Designedforlowsampleinput
- Highcoveragequality
- Acceleratedlibrarypreparation
Manualpreparationofhigh-qualitylibrariesinlessthanaday
TheprovenTruSeqDNAlibrarypreparationworkflowhasbeenstreamlinedbyreplacinggel-basedsizeselectionwithbead-basedselection,enablingresearcherstopreparehigh-qualitylibrariesinlessthanaday.Itisoptimizedforavarietyofreadlengths,from2×101bpto2×151bp.
UsewithlimitedDNAsamples
TheTruSeqNanoDNAprotocoloffersexcellentresultswithaslittleas100nginput,eliminatingthetypicalrequirementformicrogramsofDNA.ThisenablesresearcherstostudysampleswithlimitedavailableDNA(eg,tumorsamples)andhelpspreservesamplesforuseinfutureoralternatestudies.Inadditiontoacceleratingtheworkflow,bead-basedsizeselectionavoidstypicalsamplelossassociatedwithgel-basedselection.
Reducelibrarybiasandcoveragegaps
TruSeqNanoDNAkitsreducethenumberandaveragesizeoftypicalPCR-inducedgapsincoverage.Theenhancedworkflowreduceslibrarybiasandimprovescoverageuniformityacrossthegenome.ThesekitsalsoprovideexcellentcoverageoftrADItionallychallenginggenomiccontent,includingGC-richregions,promoters,andrepetitiveregions.Thisenablesresearcherstoaccessmoreinformationfromeachsequencingrun.TruSeqNanoDNAkitsarevalidatedforhigh-qualitygenomiccoverageforvirtuallyanynext-generationsequencingapplication.
AccessflexIBLethroughputoptions
Kitsincludereagents,samplepurificationbeads,andindexes,withtwooptionsforflexibility:
- TruSeqNanoDNALTLibraryPreparationKitssupport24-plexmanualprocessingforlow-throughputstudies.
- TruSeqNanoDNAHTLibraryPreparationKitsare96-plexforhigh-throughputstudies,andcanbeautomatedonliquidhandlingrobots(orprocessedmanually).
- TruSeqNanoDNALibraryPreparationKitsarealsoavailableforusewiththeautomatedNeoPrepLibraryPrepSystem.
Findanup-to-datelistofautomationvendorswithroboticsystemsthatsupporttheHTlibrarypreparationkits
Specifications:
AssayTime | 1day |
Hands-OnTime | 4hours |
InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
Multiplexing | Upto384samplespersequencingrun |
MechanismofAction | Amplification |
Method | ShotgunSequencing,Whole-GenomeSequencing,GenotypingbySequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels),CopyNumberVariants(CNVs) |
SpeciesCategory | Mammalian,Mouse,Human,Other,Rat,Plant |
SystemCompatibility | NextSeq550,HiSeq3000,HiSeqXFive,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq2000,MiSeq,HiSeqXTen,NeoPrep,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
SpecializedSampleTypes | LowInput |
Technology | Sequencing |
AutomationCapABIlity | NeoPrepDigitalMicrofluidics,LiquidHandlingRobots |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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