ProductHighlights:
High-QualityWhole-TranscriptomeAnalysiswithPreciseStrandInformation
RNA-Seqtechnologyprovidesuniformcoverage,precisemeasurementofstrandorientationandhigh-confidencediscoveryoffeaturessuchasalternativetranscripts,antisenseexpressionandallele-specificexpressionacrossbothcodingandmultipleformsofnoncodingRNA.TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitcouplesthebenefitsofTruSeqRNAlibrarypreparationwithRibo-ZeroribosomalRNAreductionchemistry,providingarobustandscalablesolutionforwhole-transcriptomeanalysis.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
EfficientRibosomalRNAReductionAcrossaRangeofPlantSpecies,StudyDesigns
TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitenablesrapidandspecificremovalofcytoplasmic,mitochondrialandchloroplastribosomalRNAfromleaf,seed,androottissue.Thekithasbeenvalidatedforuseinmultipleplantspecies,includingArABIdopsisthaliana,rice,andmaize,andwillprovideefficientrRNAremovalinabroadrangeofadditionalspecies.*
*Forinformationonaparticularspeciesofinterest,contactIlluminaTechnicalSupport.
Specifications:
AssayTime | ~6.5hours |
Hands-OnTime | ~3.5hours |
InputQuantity | 10nghigh-qualitygenomicDNA;10–100ngFFPEDNA(dependingonQCresults) |
MechanismofAction | Probehybridization,extension-ligation,andPCR |
Multiplexing | 1–96 |
ContentSpecifications | Designcustomprobestosequencegenomicregionsofinterest.Contentrange:4–650kb |
VariantClass | ShortTandemRepeats(STRs),SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels) |
SystemCompatibility | HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq1500,NextSeq500,MiSeqFGxinResearchMode,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Bovine,Mouse,Human,Rat |
SpecializedSampleTypes | FFPE,LowInput |
Technology | Sequencing |
AutomationCapability | LiquidHandlingRobots |
Method | AmpliconSequencing,CustomSequencing |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
暂无品牌分类
暂无品牌问答