ProductHighlights:
TheTruSeqStrandedmRNALibraryPrepKitforNeoPrepoffersastreamlined,cost-efficient,andscalablesolutionforcodingtranscriptomeanalysis.Itenablesrobustinterrogationofbothstandardandlow-qualitysamples.
- Simplifiedandseamlessworkflowsolution–Generatelibrarieswithminimalhands-ontime
- Unparalleledperformanceandreproducibility–InterrogategeneexpressionwithTruSeqStrandedmRNAcoverageandquality,andreduceduservariABIlity
- Lowinputrequirement–Beginlibraryprepwithaslittleas25ngtotalRNA
PreciseandAccurate
GetprecisemeasurementofmRNAstrandorientationfordetectionofantisensetranscription,enhancedtranscriptannotation,andincreasedalignmentefficiency.Highcoverageuniformityenhancesthediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Cost-Efficient
Strandedinformationidentifiesfromwhichofthe2DNAstrandsagivenRNAtranscriptwasderived.Thisinformationprovidesincreasedconfidenceintranscriptannotation,particularlyfornonhumansamples.Identifyingstrandoriginincreasesthepercentageofreadsthatalign,reducingsequencingcostspersample.
NeoPrepSystemenhancesTruSeqStrandedmRNAperformance
TheNeoPrepSystemenhancesTruSeqStrandedmRNAperformancebyprovidinghigh-qualityreproducIBLeresults,evenwithlowinputamountsofRNA.Digitalmicrofluidicstechnologypreciselymanipulatesdropletsthatperformthelibraryprepworkflow,withinthetightlycontrolledenvironmentoftheNeoPreplibrarycard.
Asimple,intuitiveworkflowdelivers16libraries,eliminatingmostmanualsteps,andreducinghands-ontimefrom~4.5hourstojust30minutes.Inaddition,digitalmicrofluidicsrequireslessRNAinput,enablingexcellentperformancefromaslittleas25ngoftotalRNA.
Specifications:
AssayTime | ~10.5hours |
Hands-OnTime | ~30minutes |
InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
MechanismofAction | Oligo-dTbeadscapturepolyAtails |
Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
SampleThroughput | Upto16samplesperNeoPreplibrarycard |
SystemCompatibility | GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,NeoPrep,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Mammalian,Bovine,Mouse,Human,Rat |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
Technology | Sequencing |
Method | mRNASequencing |
AutomationCapability | NeoPrepDigitalMicrofluidics |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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