ProductHighlights:
TruSeqTargetedRNAExpressionKitsenablehighlycustomizablemid-tohigh-plexgeneexpressionprofilingstudies.
Youcandefinepanelsof12-1,000assaystotargetindividualexons,isoforms,splicejunctions,codingSNPS(cSNPs),genefusions,andnon-codingRNAtranscripts,plusmultiplexupto384samples.Generatethedataequivalentofupto25,000individualRT-PCRassaysinasinglerun.*
Assaysincludeconvenientonlinepaneldesignandordering,arapidandsimpleworkflowstartingwithaslittleas50ngofRNA,andautomated,on-instrumentdataanalysis.
TypesofStudyDesigns
- Custompanels—selectupto1000targetsfromadatabaseofover400,000predesignedassays.
- Add-on(supplemental)content—addasecondoligopoolofupto1000targetstoapreviouslydesignedTruSeqTargetedRNAExpressioncustompanel.
StartaCustomProject
OrdercustompanelsusingDesignStudio,afree,user-friendly,web-basedtool.Simplyselecttheassaysyouneedandaddthemtoyourorder,withnodesigntime.
LogintoDesignStudio
BrowseTruSeqTargetedRNASampleData
HumanBrainReferenceRNA(HBRR),UniversalHumanReferenceRNA(UHRR),andhumanliverRNAsampleswerepreparedusingtheTruSeqTargetedRNAExpressionKit.
TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x51bpreadlengthconfiguration.Thetotalyieldwas2.04 Gbwith96.5%ofbasesatoraboveQ30.
NotethataccesstothisdatarequiresaBaseSpaceSequenceHublogin.
RegisterforBaseSpaceSequenceHub
ViewTruSeqTargetedRNAProjectData
(secondaryanalysiswithBaseSpaceApps)
*ReferstoinstrumentswithcatalognumberSY-410-1001andupgrade,orcatalognumberSY-410-1003
Specifications:
AssayTime | 1day |
Hands-OnTime | 4hours |
InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
Multiplexing | Upto384samplespersequencingrun |
MechanismofAction | Amplification |
Method | TargetedRNASequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,TranscriptVariants |
SpecializedSampleTypes | FFPE |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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