Description:
WithoutamplificationmoduleKAPAHyperPrepKits
Shiftyourworkflowintohyperdrive.
TheKAPAHyperPrepKitisaversatile,streamlinedsolutionforDNAlibrarypreparationforIllumina®sequencing.
Thenovelone-tubechemistry,containoptimallyformulatedandevolvedenzymesthatenablehigheryieldsofadapter-ligatedlibraryandloweramplificationbias.Thistranslatestohigherlibrarydiversity,lowerduplicationratesandmoreuniformcoverage,particularlyforFFPEandlow-inputsamples.
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
DownloadourAdapterandBeadCalculator
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Improvelibraryyieldsandsequencequality
- Higherlibraryyieldstranslatetogreatermolecularcomplexity
- FewercyclesofamplificationwithKAPAHiFiDNAPolymeraseresultsinlowerduplicationratesandimprovedcoverage
Createhigh-qualitylibrariesfromFFPEsamples
- Generatehigh-qualitylibrariesfrom250ngFFPEDNAorless*
- Significantlylowerduplicationratesandhighercoverage*
Completelibraryconstructioninlessthan3hours
- One-tubeworkflowwithminimalcleanupstepsreducesoveralltime,andminimizeshands-ontime
- Sample-to-libraryin<2hoursforPCR-freeworkflows,or<3hourswithamplification*
- Fewerhandlingstepsleadtoimprovedconsistencyandreproducibility
Improveperformancewithlow-inputsamples
- Generatemoresequence-qualitylibrarymoleculeswithoutincreasingadapter-dimers
- Increaseyieldswithoutinducingsizebias
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Applications:
Applications- WholeGenomeSequencing
- WholeExomeSequencing
- TargetedSequencing(custompanels)
- ChIP-seq
- AmpliconSequencing
- Methyl-seq
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto12months at-20˚C.
Kitscontain KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix
Components
ebiomall.com
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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