Description:
KAPASingle-IndexedAdapterKit,SetA+B(1.5µM)KAPAHyperPlusKits
NGSlibrarypreparation. Evolved.
TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:
- DNAfragmentationandlibraryprepin2.5hours*
- FlexIBLeDNAsampleinputfrom1ng–1µg*
- Reducedbiasandmaximizesequencecoverage*
- PCR-freeworkflows
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
Downloadour AdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Tunableandreproduciblefragmentation
- Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
- ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts
Industryleadinglibraryyields…
- Routinelyachieveconversionrates>80%from≥100nginput*
- SuperiorperformanceacrossarangeofDNAinputamounts*
- HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
…Enablesuperiorsequencingresults
- Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
- Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
Minimalsequencecoveragebias
- Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
- Equivalentperformancetomechanicalshearing*
- Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Applications:
Applications- WholeGenomeSequencing
- WholeExomeSequencing
- TargetedSequencing(custompanels)
- FFPESampleSequencing
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto6monthsat-20ºC.
KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.
Specifications
- SpecDescription
- CompatiblePlatformlluminaHiSeq®,MiSeq®,NextSeq®
- LibraryTypeDNA
- StartingMaterialgenomicDNA,FFPEDNA,PCRamplicons
- InputAmount1ng–1µg
ebiomall.com
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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