Description: TheMCNext™SYBR®FastqPCRLibraryQuantificationKitprovidesafastandreliablesolutiontodeterminethelibraryconcentrationwithadirectestimateclusterdensity.ThisdirectmeasurementmethodsignificantlysavestimeandresourcescomparingwithotherqPCRlibraryquantificationkitsonthemarket.ThisisachievedbyprovidingaconstructedlibraryratherthanasingleDNAfragmentasacontrolstandardwithknownclustergenerationdensityonIllumina®sequencingplatforms. TheMCNext™SYBR®FastqPCRLibraryQuantificationKitutilizesMCLAB’shighperformancefastPCRenzymebyaPhiXgenomebasedLibraryStandards(forseven10-folddilutions)withanaveragesizeof570bp,providingquickandaccuratequantificationwith0.0001–100pMdynamicrangein60min.Basedonthequantification,theresultcouldbedirectlyconvertedtoclusternumbersforloadingvolumereferences. TheMCNext™SYBR®FastqPCRLibraryQuantificationKitisarapidsolutionforyourlibraryconstructionQCtoaddvaluetoyourworkflowandincreaseconfidenceinyourresults.TheproprietaryPhiXLibraryStandardsconsistofagroupofIllumina®sequencing adapter-ligatedDNAfragmentsderivedfromthePhiXgenomewithwell-characterizedsequenceandwell-balancedGCcontent(50%).TousethislibraryasacontrolforlibrariesconstructedforsequencingonIllumina®sequencingplatformsisfarsuperiorthanasingleDNAfragmentascontrolstandardusedbyotherqPCRquantificationkitsonthecurrentmarket.Itcanberapidlyinterpolatedfortheestimatingclusterdensityandservesasaquantificationbaselineforsamplelibrarieswithouttheneedtohaveanothersequencingrunforaclusterdensitymeasurementpurpose.Itisanexcellentcontrolwithmeasuredclustergenerationconversionparametertoexaminethelibraryqualityandquantity,allowsyoutoquicklydetermineifanerrorisrelatedtothesamplepreparationbeforeahigh-costsequencingrun.Quantificationisfast,directandaccuratebyextrapolationagainstthestandardcurvegeneratedusingtheseven10-foldLibraryStandardsdilutions,followedbyasimpleclusternumberconversion. TheMCNext™SYBR®FastqPCRLibraryQuantificationKitisavailablein3types(RegularROX,LowROXandUniversal)basedontheinternalreferencedyepreferencesofthePCRThermocyclers.Thepackagesincludeready-to-use2XMasterMix,10XPrimerMixandPhiXLibraryStandards. Features TheMCNext™SYBR®FastqPCRLibraryQuantificationKitprovidesresearcherswithanaccurateandsensitivemethodforquantifyingNGSlibraries.
ConvenientpackagesforalltypesofrealtimePCRplatforms |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
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