Product Description
CST3 Monoclonal Antibody
Spec Sheet E-AB-22129 (PDF File)
Overview
Synonyms | AD 8,AD8,Amyloid angiopathy and cerebral hemorrhage,ARMD11,bA218C14.4 (cystatin C),bA218C14.4,Cst 3,Cst3,CST3 protein,Cystatin 3,Cystatin-3,Cystatin-C,Cystatin3,CystatinC,CYTC,Epididymis secretory protein Li 2,Gamma trace,Gamma-trace,HCCAA,HEL S 2,MGC117328,Neuroendocrine basic polypeptide,Post gamma globulin,Post-gamma-globulin |
Swissprot | P01034 |
Source | Mouse |
Reactivity | Human |
Immunogen | Recombinant Protein of Cystatin C of CST3 |
Application | WB,IHC-p,IF,ELISA |
Recommended dilution | WB 1:1000-2000, IHC 1:100-200, IF 1:50-1:200 |
Concentration | 1 mg/mL |
Clonality | Monoclonal |
Properties
Cellular localization | Secreted. |
Tissue specificity | Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. |
Clone No. | Clone:3B6 |
Isotype | IgG |
Purification | Protein A purification |
Conjugation | Unconjugated |
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
Background | As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by: Elabscience
Questions about this product? Ready to place an order? Email us at orders@iwai-chem.comor give us a call: (650) 486-1541 |
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采用6孔板接毒后,铺上低溶解度琼脂糖,第二天就出来了这些病变,正常细胞没有出现这种现象。
按说空斑应该出现多个,但我的六孔板中一个空最多也就出现了2个空斑,也不像文献报道的那样规则,而且很大!
由于没有做梯度稀释,会不会是接毒量过大造成的啊?请大侠指点,万分感谢!
2) 克隆技术使用使倾向于量繁殖现种群利用价值体,按自规律促进整种群优胜劣汰.意义说,克隆技术干扰自进化程.
3) 克隆技术种昂贵技术,需要量金钱物专业士参与,失败率非高.莉277实验唯.虽现发展更先进技术,功率能达2-3%.
4) 转基物提高疾病传染风险.例,产药物牛奶牛染病毒,种病毒能通牛奶染病
5) 克隆技术应用于体导致代遗传性状工控制.克隆技术引起争论核能否允许发育初期类胚胎进行遗传操作.伦理家所能接受.
6) 克隆技术用创造超,或拥健壮体格却智力低.且,克隆技术能够类效运用,男性失遗传意义.
7) 克隆技术家庭关系带影响巨.由父亲DNA克隆孩看作父亲双胞胎兄弟,延迟几十已.难设想,发现自另外完全复制品,(或)受
1.如果在引物的5’引物引入了T7启动子,3‘引物也引入了polyT,PCR扩增出全长片段,回收纯化之后可以用来直接做体外转录么?为什么看到文献里都是先把这个PCR片段连接到载体里面,扩增之后提取出来线性化之后,再进行体外转录。是不是转录对模板的量有要求?
2.如果使用一些带有T7启动子序列的载体比如pBluescriptSK,在启动子序列和酶切位点之间还有多余的一段序列,这段序列也会被启动子转录,这一小段多余的序列是否会对接下来的转染以及病毒拯救带来影响?
3.有人在做反向遗传么,哪些载体可以用来进行体外转录?插入片段为7.5K。
般基克隆物体直接取基包括面几步骤
提取植物或者物基组DNA(试剂盒)————做PCR需要基扩增(顺利2搞定)——扩增基做酶切——基连接想插入载体面(顺利1-2)——鉴定克隆基
步骤都商品化试剂或者试剂盒 要思路清晰做起快 1-2周能完
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