Optimal Concentration | 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions |
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Application | PCR |
The performance of Betaine Enhancer Solution is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents because of the following effects: Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level.
At the same time, Betaine has a sequence independent destabilizing effect on all DNA. The Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered.
Furthermore, Betaine aids the process of thermostable polymerases and reduces pauses in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.
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做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些?原因越详细越多越好,避免下次再出现这种状况,有没有其他战友遇到过这种情况?谢谢!
各位大神,有使用过ThermoPX2这个型号的pcr扩增仪吗?
PCR技术类似于DNA的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
普通PCR的孔所有温度是一样的。
请问PCR仪设置加样量有什么作用,我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系,pcr仪设置时设置了50微升体系会怎么样呢?帮忙回答一下。谢谢~
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