This is a multiplex assay kit manufactured by INDOOR Biotechnologies Inc. to be used for the quantitative determination of TEN of the following food allergens: Ana o 3(Cashew nut,Anacardium occidentale), Api g 1(Celery,Apium gravolens),Ara h 1 (Peanut,Arachis hypogaea), Ara h 3(Peanut,Arachis hypogaea),Ara h 6(Peanut,Arachis hypogaea),NBos d 5(Bovine,Bos domesticusβ-Lactoglobulin),Bos d 11(Milk,Bos domesticusβ-casein),Cor a 9(Hazelnut,Corylus avellana),Cyp c1(Fish parvalbumin,Cyprinus carpio),Gal d 1(Chicken egg white ovomucoid,Gallus domesticus),Gal d 2(Chicken egg white ovalbumin,Gallus domesticus),Gly m 5(Soybean,Glycine max), Jug r 1 (Walnut, Juglans regia), Ses i 1(Sesame, Sesamum indicum), Sin a 1(Yellow mustard,Sinapis alba), Shrimp Tropomyosin(D. pteronyssinustropomyosin) and Tri a 19 (Wheat, Triticum aestivum). This kit may be used for analysis of the above indoor allergens in environmental samples, such as house dust extracts or air filter samples and other biologic or environmental samples. NOTE:Please inlcuded your required plate type in the "Customer Notes" when you check out. The plate type options area 96 well filter plate, 96 well black solid bottom plate or no plate included with your MARIA kit order. | |||
Product Resources: | Example: MRA-MFX Certificate of Analysis | ||
For research and commercial use in vitro: not for human in vivo or therapeutic use. | |||
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液氮冷冻的原理与工艺:液氮是一种比较理想的制冷剂,五色透明、稍轻于水、惰性强、无腐蚀、对震动、电火花是稳定的,一个大气压下,液氮的汽化温度为—195.81t,蒸发潜热为47.9kcal/kg,由于空间技术和炼钢等工业的发展,大量的制氧副产品——氮气经过液化得到的液氮作为一种深冷冷源已经广泛应用于医药、激光、超导、食品、生物等各工业生产和科研领域。20世纪肋年代液氮制冷剂直接气化制冷修筑地下建筑工程,已成为一种新的土层冻结方法,为提高地层冻结速度开辟了新的途径。
1.先将冻存管放入4℃冰箱,约40min.
2.接着置于-20℃冰箱,约30~60min.
3.置于-80℃超低温冰箱中放置过夜.
4.置于液氮罐中长期保存.
我想问一下,如果在-80度存放时间约2天后再移入液氮罐,是否会影响细胞复苏时的存活率,谢谢
1, 液氮罐口径只有50mm,里面根本看不清,也不能伸进去大的东西捞,通过打捞的办法很难打捞起来。
2. 把液氮全倒出来的话,我担心泡沫盒承受不了这个温度,而且没有备用液氮罐,也没备用液氮,也没有负80度冰箱,万一这些倒出来的液氮气化掉了,而且口子这么小,我担心液氮灌不进去,到时候只能眼睁睁的看着细胞化掉了。
谁有这个经验啊,救救小女子吧,不胜感激啊!我要尽快把细胞弄出来!
我现在要测脑组织匀浆的SOD活性和MDA的含量,但是因为还有其他的一些指标要测,所以需要先把脑组织保存5-10天左右才有时间测.虽然这两个指标有很多人测,我查了一下资料,也问了几个人,答案各不相同,有人说是放在低温冰箱里面就可以了,液氮罐只是适用于长期保存;也有人说放低温冰箱SOD的活性容易丧失,到底该怎么样保存最合适?谢谢!
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