Bovine IgG ELISA Kit - (min. reactivity with Hu,Ms,Rb,Rt)
CATALOG # E-10GXSPP- Add to Wishlist
Details
- Bovine, Cow
- Rabbit
- ELISA
- IgG h+l
- 2-8C
- Milk, Plasma, Serum, Tissue Culture Fluids
- 3.125 ng/ml - 100 ng/ml
- 1.245 ng/ml
- 50 min.
- The principle of the double antibody sandwich Bovine IgG ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-Bovine IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, highly cross-adsorbed anti-Bovine IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of bovine IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Documents
- Data Sheet
- SDS
Kit Contents
- 1
One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody
- 2
One ELISA Kit Data Sheet
- 3
One Certificate of Analysis
- 4
One 50 mL bottle of Diluent Running Buffer
- 5
One 50 mL bottle of 20X Concentrated Wash Solution
- 6
One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer
- 7
One 12 mL vial of Chromogen-Substrate Solution
- 8
One 12 mL vial of Stop Solution
- 9
One Calibrator Vial
Citations
This product is for research use only, not for diagnostic or therapeutic use.
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进口品牌:GEA, IMA, Telstar
国内几家其实技术上都差不多
东富龙:品牌效应第一,不过这么多年研发投入了很多,质量有一定提升
舒博拉尼:以前Telstar的技术 但也被丢的差不多了 不善的管理使其产品缺乏市场竞争力
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