当前位置：世联博研（北京）科技有限公司供求商机orflo代理MOXIZ迷你细胞计数器moxiflow迷你流式细胞仪,微型自动细胞计数仪
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产品简介Orflo中品牌的MoxiFlow微型流式细胞仪及MoxiZ细胞计数仪，MoxiZ微型自动细胞计数仪配套芯片（CellCountingCassettes），MoxiZS型细胞计数芯片（MoxiZTypeSCassettes），MoxiZM型细胞计数芯片（MoxiZTypeMCassettes）详细介绍

简单介绍

世联博研（北京）科技有限公司正式做为Orflo中国代理商，主要产品包括MoxiFlow微型流式细胞仪及MoxiZ细胞计数产品系列，迷你细胞计数仪MoxiZ和掌上流式细胞仪MOXIFLOW。MoxiGOMoxiGOIIorflo，mxf002，MoxiFlowKit,USVersion

产品描述

备有库存现货，随时发货

ORFLOTechnologies总部位于美国爱达荷州，拥有多项国际先进技术，如微流控、生物信号向电子信号转化、数据采集和分析、配套分析软件设计等，致力于现代细胞分析技术创新和开发,在国际细胞分析技术竞争中处于的地位。

ORFLOTechnologies2010年开发全球*个掌上细胞计数仪，产品一经推出，很快受到业内广泛认可。目前推出代迷你细胞计数仪MoxiZ和掌上流式细胞仪MOXIFLOW。

世联博研（北京）科技有限公司正式做为Orflo中国代理商，主要产品包括MoxiFlow微型流式细胞仪及MoxiZ细胞计数产品系列等。

美国ORFLO公司推出的一台手持式微型流式细胞仪。她的外观灵巧，完全可将她托放与掌中，便于移动，让你的实验地点不再被庞大的仪器所束缚；操作便捷，无需受过专业培训就可以完成操作，因为所有操作就只有两步（PlugPlay），上机10秒后就可在彩色触屏显示器上看到实验结果，简便至极。价钱方面，对于绝大多数的实验室来说，如果想拥有她，从经济角度上讲是完全可行的。看到这里估计读者已经迫不及待的想进一步了解和认识她了，下面就由笔者向各位介绍这位灵动、聪慧的姑娘MoxiFlow(图1)。

内外兼修

对于它外观的小巧此处不再累述（大小规格仅为261515cm），图片比文字更有说服力（图2）；仪器内置4500mAh锂电池，便于在实验室内的移动和随意放置；内置软件程序，480X320pix彩色显示器可清晰呈现数据结果，让您一目了然。至于检测方式，它采用了两种检测的*融合库尔特电阻原理与荧光检测原理：

图2手持式流式细胞仪

一、库尔特原理（用于检测细胞大小及数量）
当悬浮于液体中的细胞通过小孔管时，会取代相同体积的液体，在恒定电流设计的电路中导致小孔管内外的电阻发生瞬时变化，产生电位脉冲，而这种脉冲信号的大小和次数与细胞的大小与数目成正比，因此可用于细胞计数及大小的检测。这正是谈到流式细胞仪的历史而不可避开的库尔特原理，库尔特电阻检测法属于对细胞个体的测量和三维的测量，不但能准确测量细胞的大小，更能对细胞的绝对数量及浓度进行测量。利用库尔特原理检测细胞大小更近真实，而不像激光衍射散射原理会受到染料颜色和浓度的影响。流式细胞仪（FCM,Flowcytometry）,从字面上的cytometry(细胞计数)也看得出它与细胞计数有着一定的渊源,WallaceCoulter于1947年发明了库尔特原理，用于血细胞分析，随后1953年Coulter公司便推出了世界上*台流式细胞分析仪。而以库尔特原理为主导技术开发细胞计数仪的ORFLO公司在此推出这样一台众望所归的微型流式细胞仪也便是名正言顺了，并利用其的薄膜传感器技术对细胞进行数量、直径等检测，获取更加精准可靠的数据。

二、荧光检测（用于检测荧光信号）
MoxiFlow配置了一根532nm固态激光器和一个PMT，检测光谱区域为590/40nm(可检测染料：R-PE,PI,NileRed，EthidiumHomodimerIIII,SytoxOrange等)。对细胞大小检测的同时以传统流式的方法利用激光激发标记在细胞上的特异性荧光染料，并通过PMT进行检测，获取荧光信号。

MoxiFlow即利用了以上两种检测方式相结合的方式，在细胞流经小孔管时，同时对细胞的大小、数目及荧光信号进行精确的检测（如图3）。

化繁为简

一、简易化操作
微流体系膜片内设细胞过滤功能，所以对于准备上机的细胞悬液无需进行滤网的过滤，直接即可加入膜片孔内准备实验（如图4），此外膜片的窗口处为非透明材质做成，形成小的避光暗室，避免了荧光检测过程中荧光猝灭现象；仅需两步即可完成所有操作步骤。一，将细胞微流膜片插入检测口；二，将50L样品加入检测孔内，点击Play即可开始检测（如图5~6）；仅10秒钟即可显示出实验结果。输出数据的格式为标准的流式数据格式FCS3.1或.BMP的图像输出格式，结果数据可以以散点图及直方图（如图7）的形式表现出来，并可轻松用手触摸。

二、程序化实验
MoxiFlow将较为常用的流式实验程序化为软件中的多个选项（如图8，并在不断更新升级中），在实验室过程中只需点击要进行的实验名称即可完成相应的数据检测，如：细胞活性检测、细胞凋亡检、多参数分析、细胞大小检测、荧光珠子检测、细胞周期检测（comingsoon）等，使用系统内相应实验程序即可，应用哪里，点哪里，无需再对电压、荧光补偿等参数进行调整。没有经过任何专业培训的人员也可轻松操作完成，可见MoxiFlow解放了科研人员对于流式细胞仪使用操作的繁琐性及专业性，是对流式细胞仪的一次崭新的革命。

性能表现

为了展现出MoxiFlow优秀的检测性能，下面分别将MoxiFlow与台式流式细胞仪、成像细胞分析系统、血细胞计数板进行比较。结果发现，对于细胞的计数误差要低于其他类型产品，而计数平均值的变异系数（CV）也是zui小的，可见MoxiFlow对于细胞数量的检测表现优秀(图9左)；对于细胞活性的检测误差值与平均活性检测的波动性（CV）都是zui小的，即在对细胞活性检测的实验中，MoxiFlow依然很好地保证了数据的精准度和稳定性（图9右）。

下图是MoxiFlow细胞凋亡实验的结果图，此实验是用喜树碱（一种抗癌药物）对Jurkat细胞（白血病细胞）分别刺激不同时间（2h，6h，7h）所得到的散点图。可以评测出该药物对Jurkat细胞凋亡的作用，并给出各个群落的细胞数量、细胞浓度、占细胞总数的百分比及细胞的平均直径等信息。

总结：

外形的小巧并没有影响MoxiFlow的应用性能，所谓，麻雀虽小五脏俱全。它可帮你摆脱对流式细胞仪想爱不敢爱的矛盾心理，让流式不再高不可攀，让细胞在你的掌中流动。

英文介绍：

FlowCytometers

ORFLOdeliverssimple,affordable,yetpowerfulsolutionsforflowcytometryandcellanalysis.Nowyoucanfocusyoureffortsonresearchanddrivingyourpaceofdiscovery,ratherthanallthecomplexitythatcomeswithtraditional,complicatedflowcytometers.

ORFLOhasrevolutionizedflowcytometrybyintegratingtraditionalfluorescencebasedflowcytometrywiththeCoulterPrinciple.Nowforthefirsttimedirectcellcountsandvolumearegeneratedinparallelwithfluorescence.AttheheartofORFLOsplatformsistheirdisposableflowcytometersensor,whicheliminatestheneedforcleaning,maintenance,expensiveservicecontractsandcalibration.

MoxiFlow

Worldsmostaffordableflowcytometer,idealforsinglecolorflow,cellhealthandcellQC.

LearnMore

MoxiGOMoxiGOII

IncreasethepaceofscientificdiscoverywithORFLOsnextgenerationMoxiGOMoxiGoIINextGenFlowCytometers.

LearnMore

ProductCode:MXF002

MoxiFlowinstrumentwithUSBpowercord,USstyleUSBpoweradapter,andTypeMF-Scassettepack

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Instruments

OverviewSpecificationsHowItWorksCustomerData
PlugandPlaySimplicity-MoxiFlowistheWorldsfirstSMARTFLOWCYTOMETER.

PlugandPlaySimplicity

MoxiFlowisafullyautomated,cassette-basedflowcytometerthatcombinesunparalleledeaseofusewiththeprecisionandaccuracynormallyonlyassociatedwithmoreexpensiveflowcytometers.Thisultra-smallinstrumentusespatentedmicrofluidicthin-filmcassettesthatenableautomaticloadandmeasureoperation.TheMoxiFlowisalsotheonlyflowcytometerthatauto-alignsthelasertoeachcassettetoenablehighlyrepeatableandrobustresultseverytime.NoPMTgainadjustmentisrequired.Nowarmuptimerequired.Justinsertacassette,pipetteyoursample,andreadtheresultsin10seconds.

Versatile

Selectfromoneofourstandardapplications(i.e.,CellCounts,Viability,Apoptosis)orrunthesysteminopen2ParameterFlowCytometrymodefortheultimateinassayflexibility.Thisminiatureopenplatformcanbeusedforyourmostcommoncellassayneeds,ortodevelopsophisticated,customassaysrightatyourfingertips.

AccessyourFCScompatibleDatawithUSB-on-the-Go

MoxiFlowgeneratesdatausingtheindustrystandardFCS3.1formatsoyoucanopenandanalyzeyourresultsusingyourORFLOsVESTIGOsoftware(comingsoon),oranyotherstandardizedflowcytometryanalysissoftware.DataistransferredsimplyandeasilytoaPCorMACusingamini-USBcableandtheonboardUSB-on-the-Gofunctionality.Noadditionalsoftwareisrequiredtoperformthedatatransfer.

CellCountwith95%accuracyViabilitywithPIApoptosiswithAnnexinVPrecisecell/particle(CoulterPrinciple)Bloodanalysis(RBCcounts,WBCcounts,CD4counts,etc.)SomaticCellCountsinMilk

CellCountandViability

FlowcytometrybasedviabilitytestswithsimultaneousCoulterPrincipleCellCountsandCellVolume

BelowarescreenshotstakendirectlyfromtheMoxiFlow.TheleftscreenshotisascatterplotoffluorescenceintensityofthePropidiumIodide(PI),usedtoselectfordeadcells,versuscellvolumeonthexaxis,convertedtocelldiameter.Themiddlescreenshotiscellvolumeonly,whichshowsthecellvolumeheterogeneityofthecancerlinepopulationalongwithsubtleshiftinthemeancellvolumeofthedeadcellpopulation.Therightscreenshotshowsthefluorescenceintensityofthebaselineviablecellpopulationversusthedeadcellpopulation.

PropidiumIodidewasusedtodetecttheexactcellcountofthedead,nonviablecells.Theviabilitypercentisdisplayedintheyellowtextbox.Theconcentrationandvolumeoftheviablelivecellsandnonviablecellsisdisplayedintheblacktextboxes.

Apoptosis(AnnexinV)andCellCount

BelowarescreenshotstakendirectlyfromtheMoxiFlow.TheleftscreenshotisascatterplotoffluorescenceintensityoftheAnnexinVPEversuscellvolumeonthexaxis,convertedtocelldiameter.Therightscreenshotiscellvolumeonly,whichshowsthecellvolumeheterogeneityofthecancerlinepopulation.AnnexinVconjugatedwithPEwasusedtodetecttheexactnumberofcancercellsthatwereapoptotic.Thepercentofapoptoticcellsisdisplayedintheyellowtextbox.Theconcentrationandvolumeofthenon-apoptoticandapoptoticcellsisdisplayedintheblacktextboxes.Thistogetherwiththeabovecellviabilityandcellcounttestsgivesareadofoverallcellhealth.

CellCountandTransfectionEfficiencyChecks

TransfectionEfficiencyFlowCytometery

BelowarescreenshotstakendirectlyfromtheMoxiFlow.TheleftscreenshotisascatterplotoffluorescenceintensityoftheRFP(tdTomato,DSRed,NileRed,etc.)versuscellvolumeonthexaxis,convertedtocelldiameter.Therightscreenshotisfluorescenceonly,whichshowsheterogeneitythetransfectRFPwithinthecancerlinepopulation.TheRFPinthiscasewastdTomato.Inthisexamplethereweretwodistinctpopulationsofthetransfectedcelllines.Bothare100%positivelytransfected.Itispossiblethatthiscouldbeaninidicationofcellcycle,thelowerpopulationcouldbeinG0/G1phasewithapproximayhalftheDNAoftheupperpopulation,G2/Mphase.

RapidImmunoProfilingofWhiteBloodCells,PBMCs,WholeBlood

ImmonoProfilingFlowCytometry

InalltestsshowninthefiguresbelowTonboPEconjugatedflowcytometeryantibodieswereused.

InthescreenshotsbelowthePBMCsamplewasscreenedforthenumberofCD2positiveversusCD2negativecells,usingTonbosPEAntiHumanCD2antibody(RPA-2.10).

TheRPA-2.10antibodyreactswithhumanCD2,anapproximay50kDaglycoprotein,andamemberoftheIgsuperfamily.CD2,alsoknownasLFA-2,isareceptorforCD58inthehumanandisexpressedonthecellsurfaceof80-90%ofhumanperipheralbloodlymphocytes,asubsetofNKcells,andallmatureTcells.CD2mediateslymphocyteadhesionandisinvolvedinTcellactivation.RPA-2.10isreportedtoblockmixedlymphocytereaction.Pleasechoosetheappropriateformatforeachapplication.

TheMoxiFlowFlowCytometerCellCounter(CoulterPrinciplebased)isabletoclearlyseparateouttheCD2positivecellsversustheCD2negativecells,deliveringahighlyaccurateandprecisecountofthetwopopulations.Thefolddifferencebetweenthepositiveandnegativewhitebloodcellsisasimplerelativeproteinquantitationmethod.

InthescreenshotsbelowthePBMCsamplewasscreenedforthenumberofCD3positiveversusCD3negativecells,usingTonbosPEAntiHumanCD3antibody(Hit3a).

TheHit3aantibodyisspecificforhumanCD3e,alsoknownasCD3epsilon,a20kDasubunitoftheTcellreceptorcomplex,alongwithCD3gammaandCD3delta.TheseintegralmembraneproteinchainsassemblewithadditionalchainsoftheTcellreceptor(TCR),aswellasCD3zetachain,toformtheTcellreceptorCD3complex.Togetherwithco-receptorsCD4orCD8,thecomplexservestorecognizeantigensboundtoMHCmoleculesonantigen-presentingcells.TheseinteractionspromoteTcellreceptorsignaling(Tcellactivation),inducingcellproliferation,differentiation,productionofcytokinesoractivation-inducedcelldeath.CD3isdifferentiallyexpressedduringthymocyte-to-TcelldevelopmentandonallmatureTcells.

TheHit3aantibodyisawidelyusedphenotypicMarkerforhumanTcells.Inaddition,binding/cross-linkingofHit3aantibodytoCD3ecaninducecellactivation.Theantibodyhasalsobeendemonstratedtobecross-reactivewithChimpanzeeCD3.Pleasechoosetheappropriateformatforeachapplication.

TheMoxiFlowFlowCytometerCellCounter(CoulterPrinciplebased)isabletoclearlyseparateouttheCD3positivewhitebloodcellsversustheCD3negativewhitebloodcells,deliveringahighlyaccurateandprecisecountofthetwopopulations.Thefolddifferencebetweenthepositiveandnegativewhitebloodcellsisasimplerelativeproteinquantitationmethod.

InthescreenshotsbelowthePBMCsamplewasscreenedforthenumberofCD4positive(tcells)versusCD4negativecells,usingTonbosPEAntiHumanCD4antibody(OKT4).

TheOKT4antibodyreactswithhumanCD4,a59kDaproteinwhichactsasaco-receptorfortheTcellreceptor(TCR)initsinteractionwithMHCClassIImoleculesonantigen-presentingcells.TheextracellulardomainofCD4bindstothebeta-2domainofMHCClassII,whileitscytoplasmictailprovidesabindingsiteforthetyrosinekinaselck,facilitatingthesignalingcascadethatinitiatesTcellactivation.CD4,andco-receptorsCCR5andCXCR4,mayalsobeutilizedbyHIV-1toenterTcells.HumanCD4istypicallyexpressedonthymocytes,somematureTcellpopulationssuchasTh17andTregulatory(Treg)cells,aswellasondendriticcells.TheOKT4antibodyiswidelyusedasaphenotypicmarkerforCD4expression.Itiscross-reactivewithCD4inseveralnon-humanspecies,includingChimpanzee,CynomolgusandRhesus.Thisantibodyrecognizesadifferentepitope,andthusdoesnotblockbindingof,thealternativeAnti-HumanCD4antibodycloneRPA-T4(ReinherzEL,etal.1979.Proc.Natl.Acad.Sci.76:4061-4065)

TheMoxiFlowFlowCytometerCellCounter(CoulterPrinciplebased)isabletoclearlyseparateouttheCD4positivewhitebloodcellsversustheCD4negativewhitebloodcells,deliveringahighlyaccurateandprecisecountofthetwopopulations.Thefolddifferencebetweenthepositiveandnegativewhitebloodcellsisasimplerelativeproteinquantitationmethod.

InthescreenshotsbelowthePBMCsamplewasscreenedforthenumberofCD8positiveversusCD4negativecells,usingTonbosPEAntiHumanCD8antibody(SK1).

TheSK1antibodyisspecificforthe32-34kDaalphachainofhumanCD8,knownasCD8aorCD8alpha.CD8acanformahomodimer(CD8alpha-alpha),butismorecommonlyexpressedasaheterodimerwithasecondchainknownasCD8borCD8beta.CD8actsasaco-receptorforantigenrecognitionandsubsequentTcellactivationthatisinitiateduponbindingoftheTcellreceptor(TCR)toantigen-bearingMHCClassImolecules.ThecytoplasmicdomainsofCD8providebindingsitesforthetyrosinekinaselck,facilitatingintracellularsignalingeventsthatleadtoTcellactivation,development,andcytotoxiceffectorfunctions.CD8+cytotoxicTcells(CTLs)playanimportantroleininducingcelldeathoftumorcells,aswellascellsinfectedbyvirus,bacteriaorparasites.

TheSK1antibodyiswidelyusedasaphenotypicmarkerforCD8oncytotoxicTcells,thymocytes,aswellasoncertaincelltypesthatdonotalsoexpresstheTCR,includingsomeNKcellsandlymphoiddendriticcells.Itiscross-reactivewithCD8inseveralnon-humanspecies,includingBaboon,Chimpanzee,CynomolgusandRhesus.IfusedtogetherwithanalternativeAnti-HumanCD8aclone,RPA-T8,theSK1antibodywillnotblockbindingofRPA-T8toCD8a.

TheMoxiFlowFlowCytometerCellCounter(CoulterPrinciplebased)isabletoclearlyseparateouttheCD8positivewhitebloodcellsversustheCD8negativewhitebloodcells,deliveringahighlyaccurateandprecisecountofthetwopopulations.Thefolddifferencebetweenthepositiveandnegativewhitebloodcellsisasimplerelativeproteinquantitationmethod.

InthescreenshotsbelowthePBMCsamplewasscreenedforthenumberofCD11positiveversusCD11negativecells,usingTonbosPEAntiHumanCD8antibody(ICRF44).

TheICRF44antibodyreactswithhumanCD11b,alsoknownasintegrinalphaM.This165-170kDacellsurfaceglycoproteinispartofafamilyofintegrinreceptorsthatmediateadhesionbetweencells(cell-cell)andcomponentsoftheextracellularmatrix,e.g.fibrinogen(cell-matrix).Inaddition,integrinsareactivesignalingreceptorswhichrecruitleukocytestoinflammatorysitesandpromotecellactivation.Complete,functionalintegrinreceptorsconsistofdistinctcombinationsofintegrinchainswhicharedifferentiallyexpressed.IntegrinalphaM(CD11b)assembleswithIntegrinbeta-2(CD18)intoareceptorknownasMacrophageAntigen-1(Mac-1)orcomplementreceptortype3(CR3).ThisreceptorbindsandinducesintracellularsignalingthroughICAM-1,ICAM-2,ICAM-3andICAM-4onendothelialcellsandcanalsofacilitateremovalofiC3bbearingforeigncells.

TheICRF44antibodyiswidelyusedasamarkerforCD11bexpressiononmacrophages,granulocytes,andsubsetsofNKcells.Itisreportedtobecross-reactivewithanumberofnon-humanspeciesincludingBaboon,Chimpanzee,Cynomolgus,RhesusandSwine.

TheMoxiFlowFlowCytometerCellCounter(CoulterPrinciplebased)isabletoclearlyseparateouttheCD11positivewhitebloodcellsversustheCD11negativewhitebloodcells,deliveringahighlyaccurateandprecisecountofthetwopopulations.Thefolddifferencebetweenthepositiveandnegativewhitebloodcellsisasimplerelativeproteinquantitationmethod.

InthescreenshotsbelowthePBMCsamplewasscreenedforthenumberofCD19positivebcellsversusCD19negativewhitebloodcells,usingTonbosPEAntiHumanCD19antibody(SJ25C1).

TheSJ25C1antibodyreactswithhumanCD19,a95kDaglycoproteinwhichactsasaco-receptor,alongwithCD21(CR2),CD81(TAPA-1)andCD225(Leu13),insupportofthefunctionalBcellreceptor(BCR).Thiscomplexprovidesantigen-specificrecognitionandsubsequentactivationofBcellstoproliferateanddifferentiateintoantibody-secretingcells(plasmacells)ormemoryBcells,whicharecrucialforsecondaryantigenencounter.Uponactivationandtyrosinephosphorylation,theCD19moleculecanprovideananchorforcytoplasmicsignalingproteinssuchasGRB2,SOSorPLCG2.CD19isalineage-differentiationmarker,asitsexpressionisdetectableattheearliestBcellstages,throughdevelopment,andisfinallylostupontransitiontomatureplasmacells.

TheSJ25C1antibodyiswidelyusedasaphenotypicmarkerforCD19expressiononBcells,aswellasondendriticcellsubsets.

TheMoxiFlowFlowCytometerCellCounter(CoulterPrinciplebased)isabletoclearlyseparateouttheCD19positivewhitebloodbcellsversustheCD19negativewhitebloodcells,deliveringahighlyaccurateandprecisecountofthetwopopulations.Thefolddifferencebetweenthepositiveandnegativewhitebloodcellsisasimplerelativeproteinquantitationmethod.

MoxiGOMoxiGOII
ProductCode:MXG001_MXG002_MXG102

MoxiGO532nmlaser(MXG001)
1PMT-561nm/LPfilter(e.g.PE,PI)-USVersion
MoxiGO488nmlaser(MXG002)
1PMT-User-swappable525/45nm(e.g.FITC,GFP)or561nm/LP(PE,PI)filters
MoxiGOII488nmlaser(MXG102)
2PMTsystem525/45nm(e.g.FITC,GFP)and561nm/LP(PE,PI)

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OverviewSpecificationsHowItWorks
3or4ParameterNextGenFlow

IncreasethepaceofscientificdiscoverywithORFLOsnextgenerationMoxiGoorMoxiGoIINextGenFlowCytometers.Withaone(MoxiGO)ortwo(MoxiGOII)fluorescentchannels,swappablefiltersets(MXG002-MoxiGO488nm),configurablelasertheMoxiGocanbedesignedtomeetyourlabscellanalysisneeds.Everyassayalsodeliverssimultaneouscellcountvolumedetermination,usingsingle-useflowcell.Thiseliminatesthehassleoftraditionalflowassociatedwithcleaning,maintenance,clearingofclogs,crosscontaminationandoccasionallyreplacementofbottlesandtubes.

FurthermoretheMoxiGoorMoxiGoIIuseverylittlesamplevolume,75ulsallowingyoutoconserverprecious,expensivesample,suchasstemcells.Cellconcentrationsaslowas10,000cellspermlarepossible,whichmostexperimentswouldenablealittleas5ulsofsampledilutedin70ulsofPBS.

TheMoxiGoorMoxiGoIIcanbeutilizedasanassaydevelopmentinstrumentandmanypowerfulcellbasedassayscaneasilybeoptimizedandrunonthesystem.Theseinclude:

Cellsurfaceimmuno-labelingIn-CellProteinQuantCRISPR/TransfectionOptimizationStudiesUpto4plexbeadbasedELISAsusingournewonboardMPXRelativeQuantitationApIncellWesterBlotsReactiveOxidationSpeciesExperimentsCaleinAMstudiesPhagocytosisAnalysisMitoPotentialExperiements

TheMoxiGoorMoxiGoIIcomestandardwithanultra-intuitive,plug-and-playinterfacewithfreeOSupdatesaslongasyouowntheinstrument.Nopriorflowcytometryexperienceisrequiredyousimplyjustplugandplay.

配套耗材：

一、MoxiZ微型自动细胞计数仪配套芯片(CellCountingCassettes)

芯片覆盖直径范围（2-34微米）内的所有细胞样品，无需根据细胞大小进行更换，

每个芯片可做2个测试。当样品1完成后，只需取出，将芯片的另一端插入MoxiZ，加入样品2。

在一次性盒式芯片中具有更高的性能和简单性。

MoxiZ独特的保护薄膜细胞计数盒可在8秒钟内提供高精度，可重复的细胞计数和细胞大小分析（S型盒带15秒）。受全球高端计数系统使用的金标准Coulter原理的启发，Moxi盒每个测试通过一个细胞感测区域流动数千个细胞，以准确捕获样品中每个单个细胞的基于阻抗的体积测量。基于图像的细胞计数方法，单个细胞的基于阻抗的细胞计数被证明是超过95％的准确度，而75％的准确度。

没有系统污染和集成预过滤器防止堵塞。

Moxi盒式芯片设计有独特的细胞筛，以zui大限度地减少细胞结块和堵塞。另外，Moxi盒含有100％的样品在盒体内，消除了系统污染和灭菌的可能性。

1、MoxiZS型细胞计数芯片（MoxiZTypeSCassettes）

TheTypeScassetteisidealforaccuraymeasuringmostyeastandotherparticlesassmallas3mandupto20minaveragediameterincludingsomealgaeandprotozoa.Sincethetechnologyisbasedonavolumetricmeasurement,non-sphericalparticles(14-4,200fL)canalsobemeasuredaccuray.

S型细胞计数芯片非常适用于精确测量大多数酵母和其他颗粒，平均粒径小至3m，直径达20m，包括一些藻类和原生动物。由于该技术基于体积测量，因此也可以准确测量非球形颗粒（14-4,200fL）。

CellCountingCassettes

HigherPerformanceSimplicityinOneDisposableCassette.
MoxiZsunique,patent-protectedthin-filmcellcountcassettesprovidehighlyaccurate,repeatablecellcountsandcellsizeanalysisinunder8seconds(15secondsforTypeScassette).Inspiredbythegold-standardCoulterPrincipleusedinhigh-endcountingsystemsworldwide,theMoxicassetteflowsthousandsofcellspertestthroughacellsensingzonetoaccuraycapturetheimpedance-basedvolumetricmeasurementofeachindividualcellinthesample.Impedance-basedcellcountingofindividualcellsisproventobeover95%accurateversusa75%accuracywithimage-basedcellcountingmethods.

Nosystemcontaminationandintegratedpre-filterforcloggingprevention.
Moxicassettesaredesignedwithaunique"cellsieve"tominimizecellclumpingandclogging.Inaddition,Moxicassettescontain100%ofyoursamplewithinthecassettebody,eliminatingthepossibilityforsystemcontaminationandsterilization.Cassettesaresimplyandeasilydiscardedwhentestingisfinished.

MoxiZTypeSCassettes

TheTypeScassetteisidealforaccuraymeasuringmostyeastandotherparticlesassmallas3mandupto20minaveragediameterincludingsomealgaeandprotozoa.Sincethetechnologyisbasedonavolumetricmeasurement,non-sphericalparticles(14-4,200fL)canalsobemeasuredaccuray.

MoxiZTypeSPack(25Cassettes)|MoxiZTypeSBox(250Cassettes)

MoxiZTypeMCassettes

TheTypeMcassetteisidealforaccuraymeasuringmammaliancellsorotherparticles4-25micronsinaveragediameter.Sincethetechnologyisbasedonavolumetricmeasurement,non-sphericalparticles(34-8,180fL)canalsobemeasuredaccuray.

MoxiZTypeMPack(25Cassettes)|MoxiZTypeMBox(250Cassettes)

MOXIZSystemCheckBeads(5mL)
ProductCode:MXA005

MOXIZSystemCheckBeads(5mL)

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SpecificationsidMXA005ApplicationNotesCanbeusedtocheckpropersystemfunction.BeadDiameter10micronCompatibleInstrumentsMoxiZConcentration1.1e+5beads/mLFormulationOrfloDiluentIntendedUseStatementForResearchUseOnly.ProductisnotforuseindiagnosticproceduresMaterialofConstructionPolystyreneOverallDimensions22.1mm^3SterilityNon-sterileWeight5mg

MoxiZDiluent
ProductCode:MXA006

MoxiZDiluent,100mL

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SpecificationsidMXA006ApplicationNotesMoxiZDiluentisspecialformulatedforusewiththeMoxiZsystemforoptimalsizingofparticlesandforoperationwiththeinstrumentsSmallParticleMode(SPM).ApplicationsCellDilutionApplicationsAbbreviationCdilCassetteTypesTypeM|TypeSCompatibleInstrumentsMoxiZFormulationProprietaryIntendedUseStatementForResearchUseOnly.ProductisnotforuseindiagnosticproceduresKitComponentsOne100mlbottleofdiluentNumberofTestsn/aOverallDimensions52Lx52Wx106H(mm)ProtocolPelletandresuspendcellsinMoxiZdiluentordilutesamplewith90%MoxiZdiluent.2.Pipette10uLofsampleintotube3.Pipette90uLofOrfloDiluentintotube4.Gentlymixresultingsolutiontoensurecellsareevenlymixed5.Pipette75uLofresultingsolutionintoMoxiZCassette,or50uLintoMoxiFlowcassette6.TouchscreentoruntestSampleTypeMammalianCells|LargeYeast|LargeAlgae|ProtozoaSterilityNon-SterileStorageStabilityANDHandlingStoreat2-8C.Usesteriletechniquewhenhandling.Disposeofwasteproductincompliancewithfederal,stateandlocalregulations.Donotinhaleoringest.Avoidcontactwitheyesandskin,flushaffectedareawithwaterforatleast15minutesVendorORFLOWeight152g

MoxiZDiluent,sample
ProductCode:MXA009

MoxiZDiluent,5mL

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SpecificationsidMXA009ApplicationNotesMoxiZDiluentisspecialformulatedforusewiththeMoxiZsystemforoptimalsizingofparticlesandforoperationwiththeinstrumentsSmallParticleMode(SPM).ApplicationsCellDilutionApplicationsAbbreviationCdilCassetteTypesTypeM|TypeSCompatibleInstrumentsMoxiZFormulationProprietaryIntendedUseStatementForResearchUseOnly.ProductisnotforuseindiagnosticproceduresKitComponentsOne5mlbottleofdiluentNumberofTests75OverallDimensions25Wx45H(mm)ProtocolPelletandresuspendcellsinMoxiZdiluentordilutesamplewith90%MoxiZdiluent.2.Pipette10uLofsampleintotube3.Pipette90uLofOrfloDiluentintotube4.Gentlymixresultingsolutiontoensurecellsareevenlymixed5.Pipette75uLofresultingsolutionintoMoxiZCassette,or50uLintoMoxiFlowcassette6.TouchscreentoruntestSampleTypeMammalianCells|LargeYeast|LargeAlgae|ProtozoaSterilityNon-SterileStorageStabilityANDHandlingStoreat2-8C.Usesteriletechniquewhenhandling.Disposeofwasteproductincompliancewithfederal,stateandlocalregulations.Donotinhaleoringest.Avoidcontactwitheyesandskin,flushaffectedareawithwaterforatleast15minutesVendorORFLOWeight11gOrfloAccutase
ProductCode:MXA020

OrfloAccutasecelldetachmentsolution,1X,100mL

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SpecificationsidMXA020ApplicationNotesRecommendedforthedetachmentofadherentcellsanddissociationofmammaliliancelllinesandprimarycellsApplicationsCellDetachmentApplicationsAbbreviationCdetCassetteTypesTypeM|TypeS|TypeMF-M|TypeMF-SCellTypesTestedfibroblasts|keratinocytes|vascularendothelialcells|hepatocytes|vascularsmoothmusclecells|hepatocyteprogenitors|primarychickembryoneuronalcells|bonemarrowstemcells|adherentCHOandHNKcells|macrophages|293cells|L929cells|immortalizedmousetesticulargermcells|MIC5|3T3|Vero|COS|HeLa|NT2|MG63|M24andA375metastaticmelanoma|gliomasU251|D54|HT1080fibrosarcomacells|HT29coloncancercells|Sf9insectcells|humanembryonicstemcells|humanmesenchymalstemcells|andhumanneuralstemcells.CompatibleInstrumentsMoxIZ|MoxiFlow|zEPIFlowFormulationAccutaseenzymesinDulbeccosPBScontaining0.5mMEDTAandphenolred.1Xconcentrate,nopreservatives.IntendedUseStatementForResearchUseOnly.ProductisnotforuseindiagnosticproceduresKitComponentsOne100mlbottleofcelldissassociationreagentNumberofTestsn/aOverallDimensions52Lx52Wx106H(mm)Protocol1.Place100mlAccutasebottleinroomtempwaterbathorthawovernightinfridge2.ForAdherentCells:a.Removecellmediab.Add1.5mlAccutaseper25cm2offlaskareac.incubateatRTor37Cuntilcellsdetach(typically5-15min)d.Pipettetrituratetobreakapartclusters3.Forsuspensioncellaggregates:a.Pelletcellsat300xg,5minb.Resuspendpelletin1-2mlAccutasec.Incubate15mind.Pipettetrituratetobreakapartclusters4.CountcellsontheMoxiZ,MoxiFlow,orZepiFlow(besuretodilutesamplesintostandardoperatingrangeofspecificcassette,iftheyareabovethestatedconcentrationlimits)SampleTypePrimaryMammaliancells|immortalizedadherentcells|non-EScelltypesSterilitySterileStorageStabilityANDHandlingStableat-20C.Afterthawingcanbestoredforupto2monthsat2-8C.TradenameACCUTASEisaregisteredtrademarkofInnovativeCellTechnologiesVendorORFLOWeight159g

OrfloAccumax
ProductCode:MXA021

OrfloAccumax,celldissassociationreagent,10X,100mL,sterile

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SpecificationsidMXA021ApplicationNotesRecommendedforusewiththeMoxiZandMoxiFlowplatformswhenworkingwithclumpyaggregatedcells.Itisidealforgeneratingsinglecellsuspensionsfromclumpedcellcultures,detachmentofcellsfromprimarytissueanddetachmentofcellsfromplastictissueculturware.Accumaxisspecificallydesignedforourcombinationfluorescenceampimpedancebasedflowcytometers.ApplicationsCellDetachmentApplicationsAbbreviationCdetCassetteTypesTypeM|TypeS|TypeMF-M|TypeMF-SCompatibleInstrumentsMoxIZ|MoxiFlow|zEPIFlowFormulation10Xproprietarysolutionofproteolytic,collagenolyticandDNaseenzymesanddoesnotcontainmammalianorbacterialderivedproducts.IntendedUseStatementForResearchUseOnly.ProductisnotforuseindiagnosticproceduresKitComponentsOne100mlbottleofcelldissassociationreagentNumberofTestsn/aOverallDimensions52Lx52Wx106H(mm)Protocol1.Place100mlAccumaxbottleinroomtempwaterbathorthawovernightinfridge2.ForAdherentCells:a.Removecellmediab.Add1.5mlAccumaxper25cm2offlaskareac.incubateatRTor37Cuntilcellsdetach(typically5-15min)d.Pipettetrituratetobreakapartclusters3.Forsuspensioncellaggregates:a.Pelletcellsat300xg,5minb.Resuspendpelletin1-2mlAccumaxc.Incubate15mind.Pipettetrituratetobreakapartclusters4.CountcellsontheMoxiZ,MoxiFlow,orZepiFlow(besuretodilutesamplesintostandardoperatingrangeofspecificcassette,iftheyareabovethestatedconcentrationlimits)SampleTypePrimaryMammaliancells|immortalizedadherentcells|HumanEScells|RodentESSterilitySterileStorageStabilityANDHandlingStableat-20C.Afterthawingcanbestoredforupto2monthsat2-8C.TradenameACCUMAXisaregisteredtrademarkofInnovativeCellTechnologiesVendorORFLOWeight159g

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