- Super fast protocol, optimized for same-day transfection
- Effective in a broad range of cell lines
- Ideal for high-throughput applications
- Value priced-your research dollars will go further
- Unique Advanced Conjugation Enhancement (ACE) technology promotes effective delivery with exceptionally low cytotoxicity
GenePORTER® Gold Transfection Reagent is our newest, highly effective and reproducible lipid based gene delivery formulation for the widest variety of cell lines, especially the easy-to-transfect cell lines. It utilizes our Advanced Carrier Enhancement (ACE) technology that maximizes transfection efficiency and transgene expression levels with minimal cytotoxicity. The GenePORTER Gold Reagent and protocol have been optimized to provide the fastest, easiest, and most cost effective transfection reactions possible. Because of its simplicity and cost advantage, the GenePORTER Gold Transfection Reagent is ideal for high throughput or large scale applications where the need for cost control and great results are highly desired.
GenePORTER Gold Transfection Reagent Kit
- 400 rxns 1.5 ml - T204015
- 800 rxns 2 x 1.5 ml - T204030
- 4000 rxns 15 ml - T204115
- 10 rxns sample kit 0.1 ml - T204001S
Effective molecular target delivery faces many barriers, from crossing the cell type-specific extracellular membrane, to cytoplasmic degradation, to translocation across the nuclear membrane. To be effective, the transfection reagent/DNA complexes must overcome all of these challenges while minimizing cytotoxicity. GenePORTER Gold Transfection Reagent is Genlantis" newest delivery formulation and protocol and the result of extensive R&D in the area of in vitro transfection. GenePORTER Gold Reagent is based on a proven cationic lipid formulation with more than 850 citations to date. The GenePORTER Gold kit incorporates our exclusive ACE technology, which ensures consistent delivery, high quality and reliability, and the lowest cytotoxicity of any other reagent on the market today.
Quick Protocol
GenePORTER Gold Cost Comparison
Reagent | GenePORTER Gold | Lipofectimine 2000 | Fugene | Jetprime |
Manufacturer | Genlantis | Life Technoligies / Invitrogen | Roche | Polyplus |
US Retail Price | $250 | $271 | $372 | $271 |
Reaction Number | 400 | 300 | 300 | 375 |
CostPer Reaction | $0.63 | $0.90 | $1.24 | $0.72 |
GenePORTER Gold Comparison Data (click graph to enlarge)
GenePORTER Gold Comparison Images
HEK 293 Cells (click image to enlarge)
HEK 293 Figure Legend:Transfection efficiency in HEK 293 cells. HEK 293 cells were plated on poly-D-lysine coated glass cover slips and transfected with 1ug of gWiz GFP and GenePORTER GOLD (Genlantis), jetPRIME(Polyplus-transfection) or Lipofectamine 2000(Invitrogen, Inc.) in 12 well plates according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then permeablized, and incubated with AlexaFluor� 555 phalloidin (Invitrogen, Inc.), which stains cellular actin red. Cells were mounted on slides with Prolong� Gold antifade reagent with DAPI (Invitrogen, Inc.), which stains the cell nuclei blue.Cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. Transfection efficiency, cell viability and cell morphology are simultaneously assessed indicating both high transfection efficiency and significantly higher viability for GenePORTER GOLD transfected cells relative to cells transfected with jetPRIME and Lipofectamine 2000.
RAW 264.7Cells (click image to enlarge)
RAW 264.7 Figure Legend:Transfection efficiency in hard-to-transfect RAW 264.7 cells. RAW 264.7 cells were plated on glass cover slips and transfected with 1ug of gWiz GFP and GenePORTER GOLD (Genlantis), jetPRIME (Polyplus-transfection) or Lipofectamine 2000(Invitrogen, Inc.) in 12 well plates according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen,Inc.), which stains cellular actin red. Cells were mounted on slides with Prolong Gold antifade reagent with DAPI (Invitrogen,Inc.), which stains the cell nuclei blue. Cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI.Transfection efficiency, cell viability and cell morphology are simultaneously assessed indicating both high efficiency and significantly higher viability for GenePORTER GOLD transfected cells relative to cells transfected with jetPRIME and Lipofectamine 2000.
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(2) 向盘中加减砝码时要用镊子,不能用手接触砝码,不能把砝码弄湿、弄脏;(否则,砝码会沾上污物或被手上的汗水等锈蚀砝码,造成称量结果不精确)
(3) 潮湿的物体和化学物品不能直接放到天平盘中;(否则,会腐蚀天平托盘,减少天平使用寿命)
另外,还应注意:
(4) 搬动天平时,应轻拿轻放,不可让天平受到强烈振动;(否则,会损伤天平,造成天平的称量的灵敏度和精确度降低或减少天平的使用寿命)
(5) 用毕,应将游码归零,砝码归盒;横梁用固定圈固定,保证支架刀口不受损伤(此条适用于学生天平);(否则,会损伤天平,造成天平的称量的灵敏度和精确度降低或减少天平的使用寿命。)
精确称量与天平合规性管理—张忠任2017.8.2
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我们公司是生物制药公司,最近购买了一台百万分之一天平,还未校正,想请教各位大牛,百万分之一天平如何校正,是否必须由计量局校正?制药行业有没有相关法规文件规定必须得校正?如果有,麻烦分享一下,谢谢!
我公司新购进的一台梅特勒XUS205U的十万分之一天平,他可以进行十万分之一与万分之一的转换,d值0.01mg/0.1mg跟随工程师做了四角偏差,重复性的测试,关于最小称样量工程师拒绝给做。想请教各位有没有知道怎么做的。目前了解的就是用1g的砝码做标准偏差然后反推,但是还不是很清楚怎么做
QC实验室的电子天平配有打印机,早已不是什么业界的新闻了。瑞士梅特勒-托利多公司的和德国赛多利斯等国际知名电子天平生产商在N年前(不夸张地说,20年前)就给电子天平配置了打印机,并且电子天平也可以和计算机相连接,将称量数据保存在计算机中。
我国的很多大药企和经过FDA、欧盟认证的药企,基本上都为电子天平配置了打印机和(或者)计算机,将称量数据及时打印出来,和(或者0将称量数据存储在计算机中。
电子天平配置打印机的目的是为了将打印数据及时打印出来,防止称量数据被丢失和人为的改动,或者称量数据不原始(有很多人习惯先将称量数据记录在本上或者纸上,然后在记录在正式的记录上,这也是符合TCH和cGMP的要求的。
我们为电子天平配置上打印机就能做到称量数据真实了吗?我说不一定。当然主流是操作人员会把称量数据及时真实地打印出来。非主流还是可以对称量数据可以做手脚的。为什么这样说?因为如果实验室没有LIMS系统,那么你的电子天平是独立的,操作也是独立的,没有被监视和控制。因为称量操作也会发生失败(比如称量结果超出称量范围或者称量完必,在称量物质转移时发生洒落等现象)。操作人员势必会重新进行称量,那么上次称量的数据就很可能被删除或者不打印出来。
另外,称量的时间也会被人为地改变。一是改变电子天平上的时间设置,二是改变打印机和(或者)计算机上的时间来做假。
所以,我们在给电子天平配置了打印机和(或者)计算机后,我们还应该有合适的文件或者LIMS系统设置和规定和操作人员、主管等人的权限,使我们的称量操作符合法规的要求。避免和减少差错的发生,是GMP的核心内容之一。
以上内容,仅供同行们参考,如有不妥,欢迎指正。因个人水平有限,写的内容会不全,欢迎朋友们进行补充,坛友们会感谢您的。
亲:希望我的回答能够帮助到你,如果满意,请采纳或点赞支持,给我更多助人的动力!
那位大侠有相关资料?其中原理是什么?那个最小称样量是如何得来的?
希望和大家分享这一点小小的经验。
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