Background
To simplify the use of minimal medium for fed-batch fermentations, Scarab Genomics offers medium for both the batch phase and feeding phase of fed-batch fermentations. The Korz Feed Stock supplies glucose, magnesium, iron, and trace elements for the feeding stage of Modified Korz Medium fed-batch fermentations. Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Modified Korz Minimal Medium has been extensively tested with the Scarab Clean Genome® Strains to verify its ability to support cell growth and the production of recombinant protein. Korz minimal medium is designed for high density fed-batch fermentation of E. coli (Korz et al. 1995, Sharma et al. 2007). The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements, and uses glucose as the carbon source. The same base medium used for optimizing expression in shake flasks can also be used for fed-batch fermentation, thereby providing continuity between the two processes.
Figures
Specifications
Kit Components Korz Feed Stock, 1000 ml Quality Control The media is confirmed for growth of Clean Genome® Strains using liquid culture and confirmed aseptic by no growth in liquid media and agar plates. Storage Conditions For long term storage, store the Korz Feed Stock at 4-12°C and protected from light. For short term storage the Feed Stock may be stored on the bench top at room temp for several days.
Related Products
10X Modified Korz Medium Kit, Sterile White Glove IS Detection Kit MDS™42 Chemically Competent Cell Kit MDS™42 ΔrecA Chemically Competent Cell Kit MDS™42 ΔrecA Blue Chemically Competent Cell Kit MDS™42 Combination Package Chemically Competent Cell Kit ScarabXpress® T7 lac Chemically Competent Cell Kit MDS™42 Electrocompetent Cell Kit MDS™42 ΔrecA Electrocompetent Cell Kit MDS™42 ΔrecA Blue Electrocompetent Cell Kit MDS™42 ΔrecA trfA Electrocompetent Cell Kit MDS™42 ΔrecA trfA Blue Electrocompetent Cell Kit MDS™42 Combination Package Electrocompetent Cell Kit
Support
Product Manuals Korz Feed Stock, Sterile Papers
- Korz DJ, Rinas U, Hellmuth K, Sanders EA, Deckwer WD. J Biotechnol. 1995 Feb 21;39(1):59-65. Simple fed-batch technique for high cell density cultivation of Escherichia coli.
- Sharma S, Campbell JW, Frisch D, Blattner FR, Harcum SW. Biotechnol Bioeng. 2007 Dec 1;98(5):1056-70. Expression of Two Recombinant Chloramphenicol Acetyltransferase Variants in Highly Reduced Genome Escherichia coli Strains.
- Akesson M, Karlsson EN, Hagander P, Axelsson JP, Tocaj A. Biotechnol Bioeng. 1999 Sep 5;64(5):590-8. On-line detection of acetate formation in Escherichia coli cultures using dissolved oxygen responses to feed transients.
- DeLisa MP, Li J, Rao G, Weigand WA, Bentley WE. Biotechnol Bioeng. 1999 Oct 5;65(1):54-64. Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor.
- Pósfai G, et al., (2006) Emergent properties of reduced-genome Escherichia coli. Science 312:1044-6.
Patents & Disclaimers
Products are sold for non-commercial use only, under Scarab Genomics limited use label license: Limited Label Use.Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications. Clean Genome® is a registered trademark of Scarab Genomics, LLC.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
按测量精度
可分0.2级、0.1级、0.01级或更高精度。
按仪器体积
分为笔式(迷你型)、便携式、台式还有在线连续监控测量的在线式。
根据使用的要求
笔式(迷你型)与便携式pH酸碱度计一般是检测人员带到现场检测使用。
选择pH酸碱度计的精度级别是根据用户测量所需的精度决定,而后根据用户方便使用而选择各式形状的pH计。
◆按便携性分的,分为:便携式pH计,台式pH计和笔式pH计。
◆按用途分为:实验室用pH计,工业在线pH计等。
◆按先进程度分为经济型pH计,智能型pH计,精密型pH计或分为指针式pH计,数显式pH计。
◆笔式pH计,一般制成单一量程,测量范围狭,为专用简便仪器。
便携式和台式pH计测量范围较广,常用仪器,不同点是便携式采用直流供电,可携带到现场。实验室pH计测量范围广、功能多、测量精度高。
工业用pH计的特点是要求稳定性好、工作可靠,有一定的测量精度、环境适应能力强、抗干扰能力强,具有模拟里量输出、数字通讯、上下限报警和控制功能等。向左转|向右转
进口品牌JENCO的性价比比较高。
便携式pH计主要用于现场和野外测方式,要求较高的精度和完善的功能。
实验室pH计是一种台式高精度分析仪表,要求精度高、功能全,包括打印输出、数据处理等等。
工业pH计是用于工业流程的连续测量,不仅要有测量显示功能,还要有报警和控制功能,以及安 装、清洗、抗干扰等等问题的考虑。
2.据ph计仪器精度分类:可分为0.2级、0.1级、0.05级、0.01级,数字越小,精度越高。
3.根据元器件类型分类:可分为晶体管式、集成电路式和单片机微电脑式,现在更多的是应用微电脑芯片,大大减少了仪器体积和单机成本;但芯片的开发成本很贵。
4根据读数指示分类:可分为指针式和数字显示式二种。指针式pH计现在已很少使用,但指针式仪表能够显示数据的连续变化过程,因此在滴定分析中还有使用。
5.看PH计有没有附带功能,比方说带标配RS232接口,还有一个很重的是温度补偿是自动
还是手动,自动温度补偿的PH计要比手动温度补偿的PH计要方便些,二者之间的价格你就明白了吧。
第一种是,4,7,10三种校准后,再用7测试,看这次校准是否成功。
第二种是,4,校准完就立即用4测试,看看是否校准成功,7和10也一样,我想请问下,哪种方法是正确的,合理的,为什么?急!!!
对pH值准确度要求高的小伙伴们要记得定期校正pH计哦,新一代ÄKTA纯化系统可以在线校正啦:
暂无品牌问答