Product Name | Beta - Amyloid (1 - 42), HumanDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
Size | 1 mg |
Catalog # | AS-20276 |
US$ | $295 |
Purity | % Peak Area By HPLC ≥ 95% |
Aß (1-42), a major component of amyloid plaques, accumulates in neurons of Alzheimers disease brains. Biochemical analysis of the amyloid peptides isolated from Alzheimers disease brain indicates that Aß (1-42) is the principal species associated with senile plaque amyloids, while Aß (1-40) is more abundant in cerebrovascular amyloid deposit. Solvent Information | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS) |
Storage | -20°C |
References | Ref: Nagele, R. et al. Neurosci. 110, 199 (2002); Garzon-Rodriguez, W. et al. J. Biol. Chem. 272, 21037 (1997). |
Molecular Weight | 4514.1 |
DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA | |
Sequence(Three-Letter Code) | H - Asp - Ala - Glu - Phe - Arg - His - Asp - Ser - Gly - Tyr - Glu - Val - His - His - Gln - Lys - Leu - Val - Phe - Phe - Ala - Glu - Asp - Val - Gly - Ser - Asn - Lys - Gly - Ala - Ile - Ile - Gly - Leu - Met - Val - Gly - Gly - Val - Val - Ile - Ala - OH |
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Neurotox Res 17, 421. doi: 10.1007/s12640-009-9121-2. Aksenov, MY. et al. (2010). HIV-1 protein-mediated amyloidogenesis in rat hippocampal cell cultures. Neurosci Lett 475, 174. doi:10.1016/j.neulet.2010.03.073.Van Helmond, Z. et al. (2010). Higher soluble amyloid β concentration in frontal cortex of young adults than in normal elderly or Alzheimer"s Disease. Brain Pathol 20, 787. doi: 10.1111/j.1750-3639.2010.00374.x.Nagano, T. et al. (2010). Prostaglandin E2 reduces amyloid β-induced phagocytosis in cultured rat microglia. Brain Res 1323, 11. doi: 10.1016/j.brainres.2010.01.086.Ravindran, C. et al. (2010). CpG-ODNs induces up-regulated expression of chemokine CCL9 in mouse macrophages and microglia. Cell Immunol 260, 113. doi: 10.1016/j.cellimm.2009.10.001.Ryan, D. et al. (2010). An improved method for generating consistent soluble amyloid-beta oligomer preparations for in vitro neurotoxicity studies. J Neurosci Meth 190, 171. doi: 10.1016/j.jneumeth.2010.05.001.Storace, D. et al. (2010). Elevation of β-amyloid 1-42 autoantibodies in the blood of amnestic patietns with mild cognitive impairment. Arch Neurol 67, 867. doi:10.1001/archneurol.2010.137.Tian, G. et al. (2010). Increased expression of cholesterol 24S-hydroxylase results in disruption of glial glutamate transporter EAAT2 association with lipid rafts: a potential role in Alzheimer"s disease. J Neurochem 113, 978. doi: 10.1111/j.1471-4159.2010.06661.x.Tran, TT. et al. (2010). Chronic psychosocial stress accelerates impairment of long-term memory and late-phase long-term potentiation in an at-risk model of Alzheimer"s disease. Hippocampus doi: 10.1002/hipo.20790.Vargas, T. et al. (2010). Gelsolin restores Aβ-induced alterations in choroid plexus epithelium. J Biomed Biotech 10.1155/2010/805405.Wang, Y. et al. (2010). Two-photon and time-resolved fluorescence conformational studies of aggregation in amyloid peptides. J Phys Chem B 114, 7112. doi: 10.1021/jp101496y.Wirths, O. et al. (2010). Pyroglutamate Abeta pathology in APP/PS1KI mice, sporadic and familial Alzheimers disease cases. J Neural Transmission 117, 85. doi: 10.1007/s00702-009-0314-x.Arriagada, C. et al. (2009). Apoptosis is directly related to intracellular amyloid accumulation in a cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down syndrome. Neuro Lett 470, 81. doi:10.1016/j.neulet.2009.12.062.Giliberto, L. et al. (2009). Mutant presenilin 1 increases the expression and activity of BACE1. J Biol Chem 284, 9027. doi: 10.1074/jbc.M805685200.Gu, Z. et al. (2009). β-Amyloid impairs AMPA receptor trafficking and function by reducing CA2+/calmodulin-dependent protein kinase II synaptic distribution. J Biol Chem 284, 10639. doi: 10.1074/jbc.M806508200.Guix, FX. et al. (2009). Amyloid-dependent triosphosphate isomerase nitrotyrosination induces glycation and tau fibrillation. Brain 132, 1335. doi: 10.1093/brain/awp023.Li, M. et al. (2009). Amyloid Aβ interaction with receptor for advanced glycation end products up-regulates brain endothelial CCR5 expression and promotes T cells crossing the blood-brain barrier. J Immunol 182, 5778. doi: 10.4049/jimmunol.0803013Zhao, L. et al. (2009). Macrophage-mediated degradation of Aβ-amyloid via an apolipoprotein E isoform-dependent mechanism. J Neurosci 29, 3603. doi: 10.1523/JNEUROSCI.5302-08.2009.Jimenez, S. et al. (2008). Inflammatory response in the hippocampus of PS1M146L/APP751SL mouse model of Alzheimer"s Disease: age-dependent switch in the microglial phenotype from alternative to classic. J Neurosci 28, 11650. doi: 10.1523/JNEUROSCI.3024-08.2008.Gevorkian, G. et al. (2008). Amyloid-β peptide binds to microtubule-associated protein 1B (MAP1B). Neurochem Int 52, 1030. doi:10.1016/j.neuint.2007.10.020.Solorzano-Vargas, RS. et al. (2008). Epitope mapping and neuroprotective properties of a human single chain FV antibody that binds an internal epitope of amyloid-beta 1-42. Mol Immunol 45, 881. doi: doi:10.1016/j.molimm.2007.08.008.Watanabe, T. et al. (2008). Spatial memory impairment without apoptosis induced by the combination of beta-amyloid oligomers and cerebral ischemia is related to decreased acetylcholine release in rats. J Pharmacol Sci 106, 84.Chafekar, SM. et al. (2007). Aβ 1-42 induces mild endoplasmic reticulum stress in an aggregation state-dependent manner. Antioxid Redox Signaling 9, 2245.Nelson, TJ. & DL. Alkon (2007). Protection against beta-amyloid induced apoptosis by peptides interacting with beta-amyloid. J Biol Chem 282, 31238. doi: 10.1074/jbc.M705558200.Bales, KR. et al. (2006). Cholinergic dysfunction in a mouse model of Alzheimer disease is reversed by an anti-Aβ antibody. J Clin Invest 116, 825. doi:10.1172/JCI27120.Guo, J-P. et al. (2006). Aβ and tau form soluble complexes that may promote self aggregation of both into the insoluble forms observed in Alzheimer"s disease. PNAS 103, 1953.Shaked, GM. et al. (2006). Aβ induces cell death by direct interaction with its cognate extracellular domain on APP (APP597-624). FASEB J 20, 1254.doi: 10.1096/fj.05-5032fje.Osada, Y. et al. (2005). CLAC binds to amyloid β peptides through the positively charged amino acid cluster within the collagenous domain 1 and inhibits formation of amyloid fibrils. J Biol Chem 280, 8596. doi: 10.1074/jbc.M413340200.Piccini, A. et al. (2005). β-amyloid is different in normal aging and in Alzheimer disease. J Biol Chem 280, 34186.Seidler, N. & TJ. Squire (2005). Aβ-polyacrolein aggregates: Novel mechanism of plastic formation in senile plaques. Biochem Biophys Res Communications 335, 501.Boyd-Kimball, D. et al. (2004). Role of phenylalanine 20 in Alzheimer"s amyloid β-peptide (1-42)-induced oxidative stress and neurotoxicity. Chem Res Toxicol 17, 1743.Legleiter, J. et al. (2004). Effect of different anti-Aβ antibodies on Aβ fibrillogenesis as assessed by atomic force microscopy. J Mol Biol 335, 997. doi:10.1016/j.jmb.2003.11.019.Bard, F. et al. (2003). Epitope and isotope specificities of antibodies to β-amyloid peptide for protection against Alzheimer"s disease-like neuropathology. PNAS 100, 2023. doi:10.1073/pnas.0436286100.Takata, K. et al. (2003). Role of high mobility group protein-1 (HMG1) in amyloid-β homeostasis. Biochem Biophys Res Commun 301, 699. doi:10.1016/S0006-291X(03)00024-X.Uryu, S. et al. (2003). β-amyloid-specific upregulation of stearoyl coenzyme A desaturase-1 in macrophages. Biochem Biophys Res Commun 303, 302. doi:10.1016/S0006-291X(03)00334-6.Arispe, N. & M. Doh (2002). Plasma membrane cholesterol controls the cytotoxicity of Alzheimer"s disease AβP (1-40) and (1-42) peptides. FASEB J 16, 1526. doi: 10.1096/fj.02-0829com.Kanski, J. et al. (2002). The hydrophobic environment of Met35 of Alzheimer"s Aβ(1-42) is important for the neurotoxic and oxidative properties of the peptides. Neurotox Res 10.1080/10298420290023945.Barger, SW. & AS. Basile (2001). Activation of microglia by secreted amyloid precursor protein evokes release of glutamate by cystine exchange and attenuates synaptic function. J Neurochem 76, 846. doi: 10.1046/j.1471-4159.2001.00075.x.Yao, Z-X. et al. (2001). The Ginkgo biloba extract EGb 761 rescues the PC12 neuronal cells from β-amyloid-induced cel death by inhibiting the formation of β-derived diffusible neurotoxic ligands. Brain Res 889, 181. doi: 10.1016/S0006-8993(00)03131-0.Yatin, SM. et al. (2000). Vitamin E prevents Alzheimer"s amyloid β-peptide (1-42)-induced neuronal protein oxidation and reactive oxygen species production. J Alzheimer"s Dis 2, 123.Yao, Z-X. et al. (1999). Free radicals and lipid peroxidation do not mediate β-amyloid-induced neuronal cell death. Brain Res 847, 203. doi: doi:10.1016/S0006-8993(99)02047-8.Yatin, SM. et al. (1999). Alzheimer"s amyloid β-peptide associated free radicals increase rat embryonic neuronal polyamine uptake and ornithine decarboxylase activity: protective effect of vitamin E. Neurosci Lett 263, 17. doi: 10.1016/S0304-3940(99)00101-9.Bradt, BM. et al. (1998). Complement-dependent proinflammatory properties of the Alzheimer"s disease β-peptide. J Exp Med 188, 431. doi: 10.1084/jem.188.3.431. |
ebiomall.com
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按测量精度
可分0.2级、0.1级、0.01级或更高精度。
按仪器体积
分为笔式(迷你型)、便携式、台式还有在线连续监控测量的在线式。
根据使用的要求
笔式(迷你型)与便携式pH酸碱度计一般是检测人员带到现场检测使用。
选择pH酸碱度计的精度级别是根据用户测量所需的精度决定,而后根据用户方便使用而选择各式形状的pH计。
◆按便携性分的,分为:便携式pH计,台式pH计和笔式pH计。
◆按用途分为:实验室用pH计,工业在线pH计等。
◆按先进程度分为经济型pH计,智能型pH计,精密型pH计或分为指针式pH计,数显式pH计。
◆笔式pH计,一般制成单一量程,测量范围狭,为专用简便仪器。
便携式和台式pH计测量范围较广,常用仪器,不同点是便携式采用直流供电,可携带到现场。实验室pH计测量范围广、功能多、测量精度高。
工业用pH计的特点是要求稳定性好、工作可靠,有一定的测量精度、环境适应能力强、抗干扰能力强,具有模拟里量输出、数字通讯、上下限报警和控制功能等。向左转|向右转
进口品牌JENCO的性价比比较高。
便携式pH计主要用于现场和野外测方式,要求较高的精度和完善的功能。
实验室pH计是一种台式高精度分析仪表,要求精度高、功能全,包括打印输出、数据处理等等。
工业pH计是用于工业流程的连续测量,不仅要有测量显示功能,还要有报警和控制功能,以及安 装、清洗、抗干扰等等问题的考虑。
2.据ph计仪器精度分类:可分为0.2级、0.1级、0.05级、0.01级,数字越小,精度越高。
3.根据元器件类型分类:可分为晶体管式、集成电路式和单片机微电脑式,现在更多的是应用微电脑芯片,大大减少了仪器体积和单机成本;但芯片的开发成本很贵。
4根据读数指示分类:可分为指针式和数字显示式二种。指针式pH计现在已很少使用,但指针式仪表能够显示数据的连续变化过程,因此在滴定分析中还有使用。
5.看PH计有没有附带功能,比方说带标配RS232接口,还有一个很重的是温度补偿是自动
还是手动,自动温度补偿的PH计要比手动温度补偿的PH计要方便些,二者之间的价格你就明白了吧。
第一种是,4,7,10三种校准后,再用7测试,看这次校准是否成功。
第二种是,4,校准完就立即用4测试,看看是否校准成功,7和10也一样,我想请问下,哪种方法是正确的,合理的,为什么?急!!!
对pH值准确度要求高的小伙伴们要记得定期校正pH计哦,新一代ÄKTA纯化系统可以在线校正啦:
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